NDLI: Microfluidic cell sorter with integrated piezoelectric actuator

Content Provider	Springer Nature Link
Author	Chen, Chun H. Cho, Sung Hwan Tsai, Frank Erten, Ahmet Lo, Yu Hwa
Copyright Year	2009
Abstract	We demonstrate a low-power (<0.1 mW), low-voltage (<10 Vp-p) on-chip piezoelectrically actuated micro-sorter that can deflect single particles and cells at high-speed. With rhodamine in the stream, switching of flow between channels can be visualized at high actuation frequency (~1.7 kHz). The magnitude of the cell deflection can be precisely controlled by the magnitude and waveform of input voltage. Both simulation and experimental results indicate that the drag force imposed on the suspended particle/cell by the instantaneous fluid displacement can alter the trajectory of the particle/cell of any size, shape, and density of interest in a controlled manner. The open-loop E. Coli cell deflection experiment demonstrates that the sorting mechanism can produce a throughput of at least 330 cells/s, with a promise of a significantly higher throughput for an optimized design. To achieve close-loop sorting operation, fluorescence detection, real-time signal processing, and field-programmable-gate-array (FPGA) implementation of the control algorithms were developed to perform automated sorting of fluorescent beads. The preliminary results show error-free sorting at a sorting efficiency of ~70%. Since the piezoelectric actuator has an intrinsic response time of 0.1–1 ms and the sorting can be performed under high flowrate (particle speed of ~1–10 cm/s), the system can achieve a throughput of >1,000 particles/s with high purity.
Starting Page	1223
Ending Page	1231
Page Count	9
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	11
Issue Number	6
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2009-08-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hydrodynamics Flow cytometer Microfluidics Sorter Piezoelectric actuation Engineering Fluid Dynamics Nanotechnology Biophysics and Biological Physics Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Microfluidic cell sorter with integrated piezoelectric actuator

Advanced microfluidic droplet manipulation based on piezoelectric actuation

Electrical cell counting process characterization in a microfluidic impedance cytometer

A generic label-free microfluidic microobject sorter using a magnetic elastic diverter

Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

A microfluidic localized, multiple cell culture array using vacuum actuated cell seeding: integrated anticancer drug testing

Controlling flow in microfluidic channels with a manually actuated pin valve

Microfluidic cell sorter with integrated piezoelectric actuator

A self-contained microfluidic cell culture system

Microfluidic cell sorter with integrated piezoelectric actuator

Similar Documents

Microfluidic cell sorter with integrated piezoelectric actuator

Advanced microfluidic droplet manipulation based on piezoelectric actuation

Electrical cell counting process characterization in a microfluidic impedance cytometer

A generic label-free microfluidic microobject sorter using a magnetic elastic diverter

Microfluidic sedimentation cytometer for milk quality and bovine mastitis monitoring

A microfluidic localized, multiple cell culture array using vacuum actuated cell seeding: integrated anticancer drug testing

Controlling flow in microfluidic channels with a manually actuated pin valve

Microfluidic cell sorter with integrated piezoelectric actuator

A self-contained microfluidic cell culture system

Microfluidic cell sorter with integrated piezoelectric actuator