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| Content Provider | Springer Nature Link |
|---|---|
| Author | Migita, Satoshi Hanagata, butaka Tsuya, Daiju Yamazaki, Tomohiko Sugimoto, Yoshimasa Ikoma, Toshiyuki |
| Copyright Year | 2011 |
| Abstract | Non-viral system generally demonstrates less efficacious in transgene delivery than viral system; however it represents a safer alternative to viral system. In this study, transfection efficiency for human hepatocellular liver carcinoma cells synchronized in cell cycle at G0/G1 phase, which was sorted in size with a microfluidic device based on hydrodynamic filtration, was investigated by using a reverse transfection method. The synchronized cells were recovered at the yield of 80% from the micro-channel, and green fluorescent protein gene encoding plasmid mixed with lipofectoamine was transfected. The transfection efficiency of the cells at G0/G1 phase was 1.8 times higher than non-synchronized cells. The manipulation of cell cycle status could increase transfection efficiency in non-viral system, indicating size-based cell cycle synchronization is a powerful tool as a noninvasive method for bioscience and biotechnology. |
| Starting Page | 725 |
| Ending Page | 729 |
| Page Count | 5 |
| File Format | |
| ISSN | 13872176 |
| Journal | Biomedical Microdevices |
| Volume Number | 13 |
| Issue Number | 4 |
| e-ISSN | 15728781 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2011-04-09 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cell cycle Transgene expression Microfluidic device Cell separation Engineering Fluid Dynamics Biophysics and Biological Physics Biomedical Engineering Nanotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering |
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