NDLI: A simple microfluidic device for the deformability assessment of blood cells in a continuous flow

Content Provider	Springer Nature Link
Author	Rodrigues, Raquel O. Pinho, Diana Fausti, Vera Lima, Rui
Copyright Year	2015
Abstract	Blood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of white blood cells (WBCs) are scarce despite this phenomenon often happens in the microcirculation. In this work, we present a novel integrative microfluidic device able to perform continuous separation of a desired amount of blood cells, without clogging or jamming, and at the same time, capable to assess the deformation index (DI) of both WBCs and RBCs. To determine the DI of both WBCs and RBCs, a hyperbolic converging microchannel was used, as well as a suitable image analysis technique to measure the DIs of these blood cells along the regions of interest. The results show that the WBCs have a much lower deformability than RBCs when subjected to the same in vitro flow conditions, which is directly related to their cytoskeleton and nucleus contents. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to simultaneously separate and assess blood cells deformability.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	17
Issue Number	6
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2015-10-19
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidic devices Cell separation and deformability Hyperbolic microchannel Blood on chips RBC WBC Biomedical Engineering Biophysics and Biological Physics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Extensional flow-based assessment of red blood cell deformability using hyperbolic converging microchannel

A microfluidic device for partial cell separation and deformability assessment

Gene transcript amplification from cell lysates in continuous-flow microfluidic devices

Microfluidic devices for size-dependent separation of liver cells

Lab-on-CD microfluidic platform for rapid separation and mixing of plasma from whole blood

Development of a microfluidic device for cell concentration and blood cell-plasma separation

Microfluidic analysis of pressure drop and flow behavior in hypertensive micro vessels

Sperm motion in a microfluidic fertilization device

A simple microfluidic device to study cell-scale endothelial mechanotransduction

A simple microfluidic device for the deformability assessment of blood cells in a continuous flow

Similar Documents

Extensional flow-based assessment of red blood cell deformability using hyperbolic converging microchannel

A microfluidic device for partial cell separation and deformability assessment

Gene transcript amplification from cell lysates in continuous-flow microfluidic devices

Microfluidic devices for size-dependent separation of liver cells

Lab-on-CD microfluidic platform for rapid separation and mixing of plasma from whole blood

Development of a microfluidic device for cell concentration and blood cell-plasma separation

Microfluidic analysis of pressure drop and flow behavior in hypertensive micro vessels

Sperm motion in a microfluidic fertilization device

A simple microfluidic device to study cell-scale endothelial mechanotransduction

A simple microfluidic device for the deformability assessment of blood cells in a continuous flow