NDLI: Pump-free gradient-based micro-device enables quantitative and high-throughput bacterial growth inhibition analysis

Content Provider	Springer Nature Link
Author	Ran, Min Wang, Ying Wang, Sida Luo, Chunxiong
Copyright Year	2015
Abstract	Antibiotic susceptibility testing is very important in antibiotic therapy. Traditional methods to determine antibiotic susceptibility include disk diffusion and broth dilution. However, these tests are always labor intensive, time-consuming, and need large amounts of reagents. In this paper, we demonstrated a novel pump-free micro-device that enables quantitative and high-throughput bacterial growth inhibition analysis. This device consists of a series of wells and diffusion-based antibiotic gradient channels. The wells serve as antibiotic sources and buffer sinks, and we could easily observe the bacterial growth in the gradient channels .The design of the multi-wells is adapted to the commercialized multi-channel pipette, which makes it very convenient for loading reagents into the wells. For each assay, only about 20 μL antibiotic solution is needed. As a demonstration, we used both fluorescence images and dark-field images to quantify the bacterial growth inhibition effect under different antibiotics. The quantitative data of bacterial growth inhibition under different antibiotics can be obtained within 3 to 4 h. Considering the simple operation process and the high-throughput and quantitative result this device can offer, it has great potential to be widely used in clinics and may be useful for the study of the kinetics of bacterial growth.
Starting Page	1
Ending Page	7
Page Count	7
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	17
Issue Number	4
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2015-06-05
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	High-throughput Antibiotic susceptibility testing Chemical gradient Bacterial growth inhibition Pump-free Biomedical Engineering Biophysics and Biological Physics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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