NDLI: On-chip cell lysis by antibacterial non-leaching reusable quaternary ammonium monolithic column

Content Provider	Springer Nature Link
Author	Aly Saad Aly, Mohamed Gauthier, Mario Yeow, John
Copyright Year	2015
Abstract	Reusable antibacterial non-leaching monolithic columns polymerized in microfluidic channels designed for on-chip cell lysis applications were obtained by the photoinitiated free radical copolymerization of diallyldimethylammonium chloride (DADMAC) and ethylene glycol diacrylate (EGDA) in the presence of a porogenic solvent. The microfluidic channels were fabricated in cross-linked poly(methyl methacrylate) (X-PMMA) substrates by laser micromachining. The monolithic columns have the ability to inhibit the growth of, kill and efficiently lyse Gram-positive Micrococcus luteus (Schroeter) (ATCC 4698) and Kocuria rosea (ATCC 186), and Gram-negative bacteria Pseudomonas putida (ATCC 12633) and Escherichia coli (ATCC 35218) by mechanically shearing the bacterial membrane when forcing the cells to pass through the narrow pores of the monolithic column, and simultaneously disintegrating the cell membrane by physical contact with the antibacterial surface of the column. Cell lysis was confirmed by off-chip PCR without the need for further purification. The influence of the cross-linking monomer on bacterial growth inhibition, leaching, lysis efficiency of the monolithic column and its mechanical stability within the microfluidic channel were investigated and analyzed for three different cross-linking monomers: ethylene glycol dimethacrylate (EGDA), ethylene glycol dimethacrylate (EGDMA) and 1,6-hexanediol dimethacrylate (1,6-HDDMA). Furthermore, the bonding efficiency of two X-PMMA substrates with different cross-linking levels was studied. The monolithic columns were shown to be stable, non-leaching, and reusable for over 30 lysis cycles without significant performance degradation or DNA carryover when they were back-flushed between lysis cycles.
Starting Page	1
Ending Page	13
Page Count	13
File Format	PDF
ISSN	13872176
Journal	Biomedical Microdevices
Volume Number	18
Issue Number	1
e-ISSN	15728781
Language	English
Publisher	Springer US
Publisher Date	2015-12-16
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Porous polymeric monolith Cell lysis Antibacterial Quaternary ammonium Reusable Growth inhibition Non-leaching Biomedical Engineering Biophysics and Biological Physics Nanotechnology Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Molecular Biology Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Antibacterial Porous Polymeric Monolith for On-Chip Cell Lysis

Study of a novel cell lysis method with titanium dioxide for Lab-on-a-Chip devices

Integration of nanoparticle cell lysis and microchip PCR for one-step rapid detection of bacteria

A portable pressure pump for microfluidic lab-on-a-chip systems using a porous polydimethylsiloxane (PDMS) sponge

Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips

A resettable dynamic microarray device

Polymeric nanofiber web-based artificial renal microfluidic chip

A porous 3D cell culture micro device for cell migration study

A monolithic polymeric microdevice for pH-responsive drug delivery

On-chip cell lysis by antibacterial non-leaching reusable quaternary ammonium monolithic column

Similar Documents

Antibacterial Porous Polymeric Monolith for On-Chip Cell Lysis

Study of a novel cell lysis method with titanium dioxide for Lab-on-a-Chip devices

Integration of nanoparticle cell lysis and microchip PCR for one-step rapid detection of bacteria

A portable pressure pump for microfluidic lab-on-a-chip systems using a porous polydimethylsiloxane (PDMS) sponge

Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips

A resettable dynamic microarray device

Polymeric nanofiber web-based artificial renal microfluidic chip

A porous 3D cell culture micro device for cell migration study

A monolithic polymeric microdevice for pH-responsive drug delivery

On-chip cell lysis by antibacterial non-leaching reusable quaternary ammonium monolithic column