NDLI: Proteome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.

Please wait, while we are loading the content...

Photoactivated DNA analogs of substrates of the nucleotide excision repair system and their interaction with proteins of NER-competent HeLa cell extract

Enzymatic properties of a recombinant phospholipid hydroperoxide glutathione peroxidase from Momordica charantia and its complementation function in yeast

Manganese-dependent carboanhydrase activity of photosystem II proteins

Conformational stability of cytochrome b 5, enhanced green fluorescent protein, and their fusion protein Hmwb5-EGFP

Physicochemical and kinetic characteristics of isoforms of isocitrate lyase from corn

Polysaccharides of basidiomycetes. Alkali-soluble polysaccharides from the mycelium of white rot fungus Ganoderma lucidum (Curt.: Fr.) P. Karst

Anionic polymers of the cell wall of Bacillus subtilis subsp. subtilis VKM B-501T

New isoform HvNHX3 of vacuolar Na+/H+-antiporter in barley: Expression and immunolocalization

DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences

Glycogen phosphorylase b and phosphorylase kinase binding to glycogen under molecular crowding conditions. Inhibitory effect of FAD

Isolation and properties of fungal β-glucosidases

Reversibility of nucleoside diphosphate kinase solubilization from the surface of the outer mitochondrial membrane

Biochemistry (Moscow) : Volume 74, Issue 4, April 2009

Biochemistry (Moscow) : Volume 74, Issue 3, March 2009

Biochemistry (Moscow) : Volume 74, Issue 2, February 2009

Biochemistry (Moscow) : Volume 74, Issue 1, January 2009

Biochemistry (Moscow) : Volume 73

Biochemistry (Moscow) : Volume 72

Biochemistry (Moscow) : Volume 71

Biochemistry (Moscow) : Volume 70

Biochemistry (Moscow) : Volume 69

Biochemistry (Moscow) : Volume 68

Biochemistry (Moscow) : Volume 67

Biochemistry (Moscow) : Volume 66

Biochemistry (Moscow) : Volume 65

Proteome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.

Content Provider	Springer Nature Link
Author	Skripnikov, A. Yu. Polyakov, N. B. Tolcheva, E. V. Velikodvorskaya, V. V. Dolgov, S. V. Demina, I. A. Rogova, M. A. Govorun, V. M.
Copyright Year	2009
Abstract	The sequencing of the moss Physcomitrella patens genome has facilitated studies of the plant proteome. To develop a proteome reference map based on the genome sequence, we conducted 2D electrophoreses of proteins extracted from moss protoplasts, protonemata, and gametophores grown under standard conditions on Petri dishes. On silver-stained gels, depending on the developmental stage of the moss, we resolved from 500 to 600 protein spots that were then excised and digested by trypsin, and 212 proteins were identified by PMF-MALDI-TOF. To enhance the proteome coverage, we performed 1D SDS-PAGE with subsequent separation of tryptic peptides derived from digested gel band slices by LC-ESI-MS/MS. The proposed approach allowed us to identify 186 proteins had not been determined by 2D PMF-MALDI-TOF. Proteins identified by both methods were categorized using a system of clusterization of orthologous genes as metabolism (26%), cellular processes and signaling (16%), and information storage and processing (7%). Proteome analysis by differential gel electrophoresis revealed moderate differences between filamentous protonemata and leafy shoots. Surprisingly, protoplasts isolated from protonema filaments displayed significant differences in protein composition compared with both protonemata and gametophores.
Starting Page	480
Ending Page	490
Page Count	11
File Format	PDF
ISSN	00062979
Journal	Biochemistry (Moscow)
Volume Number	74
Issue Number	5
e-ISSN	16083040
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2009-05-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	proteomics 2D electrophoresis MALDI-TOF LC-ESI-MS/MS Physcomitrella patens protoplasts DiGE Biomedicine general Microbiology Bioorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Biophysics Geriatrics and Gerontology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Proteome analysis of chloroplasts from the moss Physcomitrella patens (Hedw.) B.S.G.

The search for and identification of peptides from the moss Physcomitrella patens

Analysis of Modification of Liver Proteome in Diabetic Rats by 2D Electrophoresis and MALDI-TOF-MS

Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF

Proteomics: Emerging analytical techniques

Comparative plasma membrane-associated proteomics of immortalized human hepatocytes

Separation and study of the range of plasminogen isoforms in patients with prostate cancer

Identification of intracellular Spiroplasma melliferum metabolites by the HPLC-MS method

Proteome of the bacterium Mycoplasma gallisepticum

Proteome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.

Similar Documents

Proteome analysis of chloroplasts from the moss Physcomitrella patens (Hedw.) B.S.G.

The search for and identification of peptides from the moss Physcomitrella patens

Analysis of Modification of Liver Proteome in Diabetic Rats by 2D Electrophoresis and MALDI-TOF-MS

Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF

Proteomics: Emerging analytical techniques

Comparative plasma membrane-associated proteomics of immortalized human hepatocytes

Separation and study of the range of plasminogen isoforms in patients with prostate cancer

Identification of intracellular Spiroplasma melliferum metabolites by the HPLC-MS method

Proteome of the bacterium Mycoplasma gallisepticum

Proteome analysis of the moss Physcomitrella patens (Hedw.) B.S.G.