NDLI: DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences

Content Provider	Springer Nature Link
Author	Ignatov, K. B. Kramarov, V. M.
Copyright Year	2009
Abstract	Bacterial NAD-dependent Taq and Tth DNA ligases are capable of significantly increasing the yield of long PCR products when the amplification is carried out using bacterial family A DNA polymerases, e.g. Taq or Tth DNA polymerases, or with enzymatic blends containing these polymerases. We also show that Taq and Tth DNA ligases improve the results of PCR in the absence of NAD and therefore in the absence of DNA ligase activity. These observations suggest that bacterial DNA ligases can interact with these DNA polymerases, presumably as accessory proteins, thereby enhancing the efficiency of DNA polymerization.
Starting Page	557
Ending Page	561
Page Count	5
File Format	PDF
ISSN	00062979
Journal	Biochemistry (Moscow)
Volume Number	74
Issue Number	5
e-ISSN	16083040
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2009-05-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	long PCR Taq DNA polymerase Taq DNA ligase Biomedicine general Microbiology Bioorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Biophysics Geriatrics and Gerontology

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DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences