NDLI: Programmed Cell Death in Plants: Ultrastructural Changes in Pea Guard Cells

Content Provider	Springer Nature Link
Author	Bakeeva, L. E. Dzyubinskaya, E. V. Samuilov, V. D.
Copyright Year	2005
Abstract	Treatment with cyanide of epidermal peels isolated from pea leaves resulted in destruction of nuclei in the guard cells of stomata, which is visible with a light microscope. The process was accelerated by illumination. Electron microscopy revealed significant CN− induced changes in the ultrastructure of guard cells, which increased with time. Margination of chromatin, which is one of the first signs of apoptosis, was observed in the guard cells even after 1 h incubation of the isolated epidermis with CN−. Subsequent chromatin condensation, swelling of the endoplasmic reticulum with formation of large tanks covered with ribosomes, changes in the structure of dictyosomes, and a slight swelling of mitochondria were observed after 3 h of the epidermis incubation with CN−. After 6 h of incubation with CN−, the bulk volume of the guard cells was filled with vacuoles, the cytoplasm occupied the thin marginal layer, the nucleus was in the center similarly to the control experiment, but it was polylobal, extended in narrow cytoplasmic bands, and, despite the loss of the nuclear envelope integrity, appeared to be a self-dependent structure. In the envelope-free open regions of the nucleus, mitochondria and chloroplasts directly contacted with chromatin. Much like the cell nucleus, chloroplasts lost the integrity of the membrane, but did not swell and retained the stroma and integrity of the thylakoid system. An antioxidant di-tert-butyl-4 hydroxytoluene prevented ultrastructural changes in the cells observed after 6 h of incubation with CN−. Thus, the CN− induced death of the guard cells of stomata occurs through the mechanism of apoptosis.
Starting Page	972
Ending Page	979
Page Count	8
File Format	PDF
ISSN	00062979
Journal	Biochemistry (Moscow)
Volume Number	70
Issue Number	9
e-ISSN	16083040
Language	English
Publisher	Nauka/Interperiodica
Publisher Date	2005-01-01
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	programmed cell death apoptosis guard cells ultrastructure nucleus mitochondria chloroplasts antioxidants Pisum sativum L. Biomedicine general Bioorganic Chemistry Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Biophysics Geriatrics and Gerontology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Programmed cell death in plants: Effect of protein synthesis inhibitors and structural changes in pea guard cells

Reactive oxygen species in programmed death of pea guard cells

H2O2 intensifies CN−-induced apoptosis in pea leaves

Effect of Ca2+ on programmed death of guard and epidermal cells of pea leaves

Death of stoma guard cells in leaf epidermis under disturbance of energy provision

Chitosan-induced programmed cell death in plants

Programmed cell death in plants

Programmed cell death in plants: Protective effect of mitochondrial-targeted quinones

Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings

Programmed Cell Death in Plants: Ultrastructural Changes in Pea Guard Cells

Similar Documents

Programmed cell death in plants: Effect of protein synthesis inhibitors and structural changes in pea guard cells

Reactive oxygen species in programmed death of pea guard cells

H2O2 intensifies CN−-induced apoptosis in pea leaves

Effect of Ca2+ on programmed death of guard and epidermal cells of pea leaves

Death of stoma guard cells in leaf epidermis under disturbance of energy provision

Chitosan-induced programmed cell death in plants

Programmed cell death in plants

Programmed cell death in plants: Protective effect of mitochondrial-targeted quinones

Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings

Programmed Cell Death in Plants: Ultrastructural Changes in Pea Guard Cells