NDLI: Analysis of DNA cytosine methylation patterns in maize hybrids and their parents

Content Provider	Springer Nature Link
Author	Sun, L. F. Liu, T. J. Shan, X. H. Su, S. Z. Li, S. P. Yuan, Y. P. Zhang, J.
Copyright Year	2015
Abstract	In order to better understand the molecular basis of heterosis in maize, the methylation-sensitive amplification polymorphism method was used to estimate patterns of cytosine methylation in seedling roots and leaves and 15-d postfertilization embryo and endosperm tissues of hybrids and their parental lines Zheng58 and Chang7-2. In all tissues, total relative methylation levels in the hybrids were lower than corresponding mid-parent values, with a higher number of demethylation events inferred for the hybrids. The trend of reduced methylation and increased demethylation in the hybrids relative to their parents may allow de-repression and possibly the expression of various genes associated with a hybrid phenotypic variation. To further investigate observed methylation pattern changes, we sequenced 50 differentially displayed DNA fragments. The BLAST analysis revealed that 13 fragments shared similarity with known functional proteins in maize or other plant species including proteins related to metabolism, transposons/retrotransposons, development, stress response, and signal transduction. The genes associated with these proteins may thus contribute significantly to maize hybrid vigour.
Starting Page	266
Ending Page	272
Page Count	7
File Format	PDF
ISSN	00063134
Journal	Biologia Plantarum
Volume Number	59
Issue Number	2
e-ISSN	15738264
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-07-31
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture

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