NDLI: Characterization of genes coding phenylalanine ammonia lyase and chalcone synthase in four Pogostemon cablin cultivars

Content Provider	Springer Nature Link
Author	Zeng, S. Ouyang, P. Mo, X. Wang, Y.
Copyright Year	2015
Abstract	Pogostemon cablin (Blanco) Benth is a medicinal plant rich in terpenoids and flavonoids. Currently, the molecular mechanism of flavonoid biosynthesis in P. cablin remains unclear. In this study, three copies of the phenylalanine ammonia lyase (PAL) gene designated as PcPAL1 — PcPAL3, one copy of the chalcone synthase (CHS) gene referred to as PcCHS, and four copies of the CHS-like gene referred to as PcCHSL1 — PcCHSL4 were isolated from P. cablin. PcPAL1, PcPAL2, and PcPAL3 comprised 2 136, 2 136, and 2 148 bp full-length open reading frames (ORFs) encoding 711, 711, and 715 amino acids, respectively. PcCHS, PcCHSL1, PcCHSL2, PcCHSL3, and PcCHSL4 contained 1 173, 1 176, 1 179, 1 173, and 1 170 bp ORFs encoding 390, 391, 392, 390, and 389 amino acids, respectively. A phylogenetic analysis indicates that PcPAL2 was closer to PcPAL3 than PcPAL1, and that PcCHS was clustered with other plant CHS genes but independently to the PcCHSLs subgroup. A sequence analysis shows that PcCHSLs diverged in functional residues when compared to plant CHSs including PcCHS, suggesting that PcCHSLs may be divergent in tertiary structures. A quantitative reverse-transcription polymerase chain reaction analysis indicates that the expression profiles of PcPALs, PcCHS, and PcCHSLs in leaves and stems differed among four cultivars. Our results suggest that divergence in sequence and expression profiles of the tested genes may contribute to the diversity in flavonoids among the four cultivars.
Starting Page	298
Ending Page	304
Page Count	7
File Format	PDF
ISSN	00063134
Journal	Biologia Plantarum
Volume Number	59
Issue Number	2
e-ISSN	15738264
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-07-31
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture

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