NDLI: Bovine milk lactoferrin induces synthesis of the angiogenic factors VEGF and FGF2 in osteoblasts via the p44/p42 MAP kinase pathway

Content Provider	Springer Nature Link
Author	Nakajima, Kei ichi Kan, Yosuke Nakamura, Masato Gao, Xiao Dong Kawamura, Asami Itoh, Fumiaki Ishisaki, Akira
Copyright Year	2011
Abstract	Lactoferrin (LF) belongs to the transferrin family and is present in several physiological fluids, including milk and colostrum. LF has recently been identified as an anabolic factor for bone. Here we investigated whether bovine LF (bLF) induces synthesis of angiogenic factors by osteoblasts. If so, we examined the underlying mechanism. We found that bLF purified from milk increased the mRNA expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF2) in murine osteoblast-like MC3T3-E1 cells and primary murine osteoblasts in a time- and dose-dependent manner. Furthermore, bLF increased VEGF and FGF2 protein levels in MC3T3-E1 cells. In addition, treatment of MC3T3-E1 cells with bLF rapidly induced phosphorylation of p44/p42 mitogen-activated protein (MAP) kinase. The bLF-mediated increases in VEGF and FGF2 mRNA and protein were inhibited by U0126, a specific inhibitor of the upstream kinase that activates p44/p42 MAP kinase (MEK). Taken together, our results strongly suggest that bLF induces VEGF and FGF2 synthesis in a p44/p42 MAP kinase-dependent manner in MC3T3-E1 cells.
Starting Page	847
Ending Page	856
Page Count	10
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	24
Issue Number	5
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-03-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	FGF2 Lactoferrin Osteoblasts p44/p42 MAP kinase VEGF Physical Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Bovine lactoferrin interacts with cable pili of Burkholderia cenocepacia

Proteolytic activity of bovine lactoferrin

Proteolytic activity of bovine lactoferrin

Influence of bovine lactoferrin on selected probiotic bacteria and intestinal pathogens

Cancer prevention by bovine lactoferrin: from animal studies to human trial

Bovine lactoferrin inhibits Influenza A virus induced programmed cell death in vitro

Expression of delta-lactoferrin induces cell cycle arrest

Delta-lactoferrin induces cell death via the mitochondrial death signaling pathway by upregulating bax expression

Safety of milk and milk derivatives in relation to BSE: the lactoferrin example

Bovine milk lactoferrin induces synthesis of the angiogenic factors VEGF and FGF2 in osteoblasts via the p44/p42 MAP kinase pathway

Similar Documents

Bovine lactoferrin interacts with cable pili of Burkholderia cenocepacia

Proteolytic activity of bovine lactoferrin

Proteolytic activity of bovine lactoferrin

Influence of bovine lactoferrin on selected probiotic bacteria and intestinal pathogens

Cancer prevention by bovine lactoferrin: from animal studies to human trial

Bovine lactoferrin inhibits Influenza A virus induced programmed cell death in vitro

Expression of delta-lactoferrin induces cell cycle arrest

Delta-lactoferrin induces cell death via the mitochondrial death signaling pathway by upregulating bax expression

Safety of milk and milk derivatives in relation to BSE: the lactoferrin example

Bovine milk lactoferrin induces synthesis of the angiogenic factors VEGF and FGF2 in osteoblasts via the p44/p42 MAP kinase pathway