NDLI: The ArsD As(III) metallochaperone

Content Provider	Springer Nature Link
Author	Abdul Ajees, A. Yang, Jianbo Rosen, Barry P.
Copyright Year	2010
Abstract	Arsenic, a toxic metalloid widely existing in the environment, causes a variety of health problems. The ars operon encoded by Escherichia coli plasmid R773 has arsD and arsA genes, where ArsA is an ATPase that is the catalytic subunit of the ArsAB As(III) extrusion pump, and ArsD is an arsenic chaperone for ArsA. ArsD transfers As(III) to ArsA and increases the affinity of ArsA for As(III), allowing resistance to environmental concentrations of arsenic. Cys12, Cys13 and Cys18 in ArsD form a three sulfur-coordinated As(III) binding site that is essential for metallochaperone activity. ATP hydrolysis by ArsA is required for transfer of As(III) from ArsD to ArsA, suggesting that transfer occurs with a conformation of ArsA that transiently forms during the catalytic cycle. The 1.4 Å x-ray crystal structure of ArsD shows a core of four β-strands flanked by four α-helices in a thioredoxin fold. Docking of ArsD with ArsA was modeled in silico. Independently ArsD mutants exhibiting either weaker or stronger interaction with ArsA were selected. The locations of the mutations mapped on the surface of ArsD are consistent with the docking model. The results suggest that the interface with ArsA involves one surface of α1 helix and metalloid binding site of ArsD.
Starting Page	391
Ending Page	399
Page Count	9
File Format	PDF
ISSN	09660844
Journal	BioMetals
Volume Number	24
Issue Number	3
e-ISSN	15728773
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-12-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arsenic ArsD Metallochaperone ArsA ATP-driven efflux pump Biochemistry Physical Chemistry
Content Type	Text
Resource Type	Article
Subject	Metals and Alloys Biochemistry, Genetics and Molecular Biology Biomaterials Agricultural and Biological Sciences

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Mutations in the ArsA ATPase that restore interaction with the ArsD metallochaperone

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