NDLI: Feasibility of using a particle counter or flow-cytometer for bacterial enumeration in the assimilable organic carbon (AOC) analysis method

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Feasibility of using a particle counter or flow-cytometer for bacterial enumeration in the assimilable organic carbon (AOC) analysis method

Content Provider	Springer Nature Link
Author	Aggarwal, Srijan Jeon, Youchul Hozalski, Raymond M.
Copyright Year	2015
Abstract	Assimilable organic carbon (AOC) is one of the major determinants of microbial growth and stability in drinking water distribution systems. Nevertheless, AOC measurements are rarely conducted in practice owing, in part, to the tedious and time-consuming nature of the bioassay. Herein, we compared three alternative cell count approaches [flow cytometry with staining (FC-S), flow cytometry without staining (FC-NS), and particle counting (Coulter counter; CC)] for bacterial enumeration as a means to expedite the AOC bioassay. Our results suggest that of the three methods only FC-S provides a suitable alternative to plate counting for rapid and accurate enumeration of both P17 and NOX in the AOC bioassay. While the cell counts obtained by FC-NS were linearly correlated with those obtained using the traditional heterotrophic plate count (HPC) method (FC-NS: R2 = 0.89–0.96), the AOC values obtained by FC-NS were overestimated by 18–57 %. The CC approach was unsuccessful in enumerating Spirillum strain NOX cells because of the relatively small size of that organism. The CC counts were linearly correlated with HPC for Pseudomonas fluorescens strain P-17 (P17) cells (R2 = 0.83) but like FC-NS, the CC approach also overestimated the AOC values (for P-17). The advantage of the FC-S method over the other two is improved sensitivity and the ability to specifically enumerate whole cells (and likely viable) as opposed to non-viable cells, cell debris, and other contaminating particles introduced by the test water itself or sample handling.
Starting Page	387
Ending Page	397
Page Count	11
File Format	PDF
ISSN	09239820
Journal	Biodegradation
Volume Number	26
Issue Number	5
e-ISSN	15729729
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-07-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Flow cytometry Cell counts Biofilm Distribution system Organic carbon Bacterial growth Microbiology Soil Science & Conservation Geochemistry Terrestrial Pollution Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Waste Management/Waste Technology
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Environmental Engineering Bioengineering Microbiology

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