NDLI: A new cloning strategy for generating multiple repeats of a repetitive polypeptide based on non-template PCR

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A new cloning strategy for generating multiple repeats of a repetitive polypeptide based on non-template PCR

Content Provider	Springer Nature Link
Author	Chu, Hun Su Ryum, Jung Park, Seo Young Kim, Byung Gee Kim, Dong Myung Won, Jong In
Copyright Year	2011
Abstract	A new cloning method for generating multiple repeats of amino acids is described which can be used as biomaterials, protein polymers and biomedical applications. Although several traditional methods for cloning multiple repeats are still exploited, these are laborious and complicated because they must go through several consecutive cloning steps. To solve these problems, synthetic gene libraries encoding repetitive patterns were constructed by using non-template PCR. As a result, a ‘length library’ with fourteen different ELP repeating genes was constructed and expressed in a cell-free protein synthesis system. These results showed our novel cloning method is efficient, and has the potential capacity for synthesizing repetitive genes by PCR to be cloned in any commercial expression vectors.
Starting Page	977
Ending Page	983
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	33
Issue Number	5
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-01-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Elastin-like polypeptide (ELP) Length library Multiple repeats of amino acids Non-template PCR Biotechnology Microbiology Biochemistry Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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