NDLI: Cloning and sequence analysis of a novel xylanase gene, Auxyn10A, from Aspergillus usamii

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Cloning and sequence analysis of a novel xylanase gene, Auxyn10A, from Aspergillus usamii

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Cloning and sequence analysis of a novel xylanase gene, Auxyn10A, from Aspergillus usamii

Content Provider	Springer Nature Link
Author	Wang, Junqing Zhang, Huimin Wu, Minchen Tang, Cunduo
Copyright Year	2011
Abstract	A full-length cDNA sequence, encoding a novel endo-1,4-β-d-xylanase (AuXyn10A) of Aspergillus usamii, was obtained by using rapid amplification of cDNA ends (RACE) methods and cloned into the pUCm-T vector, followed by DNA sequencing. The cDNA gene, designated as Auxyn10A, is 1,235 bp in length harboring 5′- and 3′-non-encoding regions, as well as an ORF of 984 bp that encodes a 19-aa signal peptide, a 6-aa propeptide and a 302-aa mature peptide with a calculated MW of 32,756 Da. The AuXyn10A displays high similarity to the xylanases of Aspergillus niger, Aspergillus kawachii and Aspergillus niger, members of the glycoside hydrolase family 10. Its three-dimensional structure was predicted using http://swiss-model.expasy.org/on-line programs based on the crystal structure of Penicillium simplicissimum xylanase (1B30_A) from the family 10. The complete DNA gene was cloned from the genomic DNA of A. usamii using conventional PCR and hairpin structure-mediated PCR techniques. The DNA gene is 2,255 bp in length, containing a 510 bp of 5′-flanking promoter region and a 1,745 bp of downstream fragment that consists of ten exons and nine short introns ranging from 52 to 62 bp.
Starting Page	1029
Ending Page	1038
Page Count	10
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	33
Issue Number	5
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-01-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aspergillus usamii Gene cloning Hairpin structure Sequence analysis Xylanase Biotechnology Microbiology Biochemistry Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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