NDLI: Production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus Mortierella alpina

Content Provider	Springer Nature Link
Author	Hao, Danhui Chen, Haiqin Hao, Guangfei Yang, Bo Zhang, Baixi Zhang, Hao Chen, Wei Chen, Yong Q.
Copyright Year	2015
Abstract	To increase the commercial value of oleaginous fungus Mortierella alpina by incorporation of trans-10,cis-12 conjugated linoleic acid (CLA) into the polyunsaturated fatty acids (PUFAs) of M. alpina via Propionibacterium acnes isomerase (PAI) conversion.The PAI gene and the codon optimization version were heterologously expressed in M. alpina via Agrobacterium tumefaciens-mediated transformation (ATMT). Coding usage modification significantly improved the translation of PAI transcripts and trans-10,cis-12 CLA was produced up to 1.2 mg l−1, which corresponds to approx. 0.05 % of the total fatty acid (TFA). Since PAI prefers free linoleic acid as a substrate rather than any other forms, 5 μM long-chain acyl CoA synthetase inhibitor was added and the trans-10,cis-12 CLA content increased approx. 24-fold to 29 mg l−1, reaching up to 1.2 % (w/w) of the TFA in M. alpina.Heterologous expression of PAI in M. alpina by ATMT methods is a practicable way in biosynthesis of CLA and this system may be a feasible platform for industrial production of CLA.
Starting Page	1983
Ending Page	1992
Page Count	10
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	37
Issue Number	10
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-06-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Conjugated linoleic acid Heterologous expression Linoleic acid isomerase: Mortierella alpine Trans-10,cis-12CLA Triacsin Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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