NDLI: High-level expression, purification and characterization of carbazole dioxygenase, a three components dioxygenase, of Pseudomonas GBS.5

Content Provider	Springer Nature Link
Author	Khan, Samiya Adhikari, D. K. Gupta, Sanjay Gupta, Nidhi
Copyright Year	2015
Abstract	To investigate the conversion of carbazole into 2′-aminobiphenyl-2,3-diol using carbazole dioxygenase (CARDO) that is a multicomponent enzyme consisting of homotrimeric terminal oxygenases (CarAa), a ferredoxin (CarAc) and a ferredoxin reductase (CarAd) unit, encoded by the carAa, carAc and carAd genes, respectively.The enzyme subunits containing a GST tag were expressed independently in E. coli. The expressed proteins were purified by one-step immobilized affinity chromatography and three purified proteins could reconstitute the CARDO activity in vitro and showed activity against carbazole as well as against wide range of polyaromatic compounds.This method provides an efficient way to obtain an active carbazole dioxygenase with high yield, high purity and with activity against a wide range of polyaromatic compounds.
Starting Page	1945
Ending Page	1952
Page Count	8
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	37
Issue Number	10
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-07-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biorefining Carbazole Dioxygenase Fuel biorefining GST-tag fusion Polyaromatic compounds Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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