NDLI: Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Please wait, while we are loading the content...

Influence of hydrophilic amino acids and GC-content on expression of recombinant proteins used in vaccines against foot-and-mouth disease virus in Escherichia coli

Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells

Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Disruption of hex1 in Trichoderma atroviride leads to loss of Woronin body and decreased tolerance to dichlorvos

Construction and optimization of a Pseudomonas putida whole-cell bioreporter for detection of bioavailable copper

Biotechnological potential of endophytic actinomycetes associated with Asteraceae plants: isolation, biodiversity and bioactivities

Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator

Cloning and characterization of a protein elicitor Sm1 gene from Trichoderma harzianum

Glycoconjugates synthesized via transglycosylation by a thermostable α-glucosidase from Thermoplasma acidophilum and its glycosynthase mutant

Recombinant expression and characterization of an acid-, alkali- and salt-tolerant β-1,3-1,4-glucanase from Paenibacillus sp. S09

Molecular cloning and characterization of the α-chain gene of goose immunoglobulin heavy chain

Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain

Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment

Comparison of firefly luciferase and NanoLuc luciferase for biophotonic labeling of group A Streptococcus

A one-pot system for production of l-2-aminobutyric acid from l-threonine by l-threonine deaminase and a NADH-regeneration system based on l-leucine dehydrogenase and formate dehydrogenase

Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors

Rapid induction of the triterpenoid pathway in Arabidopsis thaliana mesophyll protoplasts

Modification to the injection needle to a screw needle improves effective cell delivery in acute myocardial infarction

Biotechnology Letters : Volume 36, Issue 3, March 2014

Biotechnology Letters : Volume 36, Issue 2, February 2014

Biotechnology Letters : Volume 36, Issue 1, January 2014

Biotechnology Letters : Volume 35

Biotechnology Letters : Volume 34

Biotechnology Letters : Volume 33

Biotechnology Letters : Volume 32

Biotechnology Letters : Volume 31

Biotechnology Letters : Volume 30

Biotechnology Letters : Volume 29

Biotechnology Letters : Volume 28

Biotechnology Letters : Volume 27

Biotechnology Letters : Volume 26

Biotechnology Letters : Volume 25

Biotechnology Letters : Volume 24

Biotechnology Letters : Volume 23

Biotechnology Letters : Volume 22

Biotechnology Letters : Volume 21

Biotechnology Letters : Volume 20

Biotechnology Letters : Volume 19

Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Content Provider	Springer Nature Link
Author	Wilde, Monika Klausberger, Miriam Palmberger, Dieter Ernst, Wolfgang Grabherr, Reingard
Copyright Year	2013
Abstract	Purpose of work: A comparative analysis of new and established insect cell lines, in regard to process relevant parameters, provide data that can be exploited for designing more robust and effective protein production processes. The baculovirus-insect cell expression system has been efficiently used for the production of heterologous proteins. Three different insect cell lines Tnao38, High Five and Sf9 were compared in terms of virus susceptibility, baculovirus production and product yield of an intra-cellularly (YFP) and extra-cellularly (influenza A virus hemagglutinin)-expressed recombinant protein. The Tnao38 and High Five cell lines exhibited higher (tenfold) susceptibility to baculovirus infection than Sf9 cells, whereas Sf9 cells showed a higher (100-fold) capacity for production of infectious virus particles. Analysis of recombinant protein expression revealed considerably higher product yields in Tnao38 and High Five cells as compared to Sf9 cells, for both model proteins. Overall, the two Trichoplusia ni-derived cell lines, High Five and Tnao38, were significantly more efficient in terms of secreting proteins such as the glycoprotein hemagglutinin of influenza A virus.
Starting Page	743
Ending Page	749
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	36
Issue Number	4
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-12-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Autographa californica nuclear polyhedrosis virus Baculovirus expression vector system Baculovirus titer determination Hemagglutinin of influenza virus Host–virus interactions Influenza viral protein Insect cell lines Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Viral and host cellular transcription in Autographa californica nuclear polyhedrosis virus-infected gypsy moth cell lines.

Concentrating Autographa californica nuclear polyhedrosis virus and recombinant alkaline phosphatase from insect cells using a temperature-sensitive hydrogel.

Encoding gp4l, a structural glycoprotein of the baculovirus autographa californica nuclear polyhedrosis virus (1992).

A baculovirus dual expression vector derived from the Autographa californica nuclear polyhedrosis virus polyhedrin and p10 promoters: co-expression of two influenza virus genes in insect cells.

baculovirus Autographa californica multiple nuclear polyhedrosis virus (2016)

Screening of High-permissive Silkworm Strains for Efficient Recombinant Protein Production in Autographa californica Nuclear Polyhedrosis Virus (AcNPV)

A baculovirus expression vector derived from the basic protein promoter of Autographa californica nuclear polyhedrosis virus.

Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Similar Documents

Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Viral and host cellular transcription in Autographa californica nuclear polyhedrosis virus-infected gypsy moth cell lines.

Concentrating Autographa californica nuclear polyhedrosis virus and recombinant alkaline phosphatase from insect cells using a temperature-sensitive hydrogel.

Encoding gp4l, a structural glycoprotein of the baculovirus autographa californica nuclear polyhedrosis virus (1992).

A baculovirus dual expression vector derived from the Autographa californica nuclear polyhedrosis virus polyhedrin and p10 promoters: co-expression of two influenza virus genes in insect cells.

baculovirus Autographa californica multiple nuclear polyhedrosis virus (2016)

Screening of High-permissive Silkworm Strains for Efficient Recombinant Protein Production in Autographa californica Nuclear Polyhedrosis Virus (AcNPV)

A baculovirus expression vector derived from the basic protein promoter of Autographa californica nuclear polyhedrosis virus.

Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression