NDLI: Molecular cloning and characterization of the α-chain gene of goose immunoglobulin heavy chain

Content Provider	Springer Nature Link
Author	Guo, Yongli Gao, Mingchun Luo, Xiuxin Sheng, Qiaoling Ma, Bo Wang, Junwei
Copyright Year	2013
Abstract	A novel gene encoding the α-chain of goose immunoglobulin heavy-chain (Igα) was cloned by reverse transcription-PCR. The cDNA had 1,760 bp and encompassed a partial V–D–J region of the heavy chain, a constant region (Cα) and 3′-untranslated region of α-chain. The Cα gene contains four constant region domains (CH1–CH4). Phylogenetic analysis indicated that goose IgCα has a close genetic relationship with duck, ostrich and chicken IgCα. Three-dimensional modeling and glycosylation analysis revealed the goose Igα is consistent with the characterization of immunoglobulin. Western blotting suggested the goose IgCα has the same antigenicity to natural IgA. In general, the identification of goose immunoglobulin not only provides insights into the evolution of the Ig heavy-chain gene family, but may also benefit future studies of the avian immune system.
Starting Page	805
Ending Page	811
Page Count	7
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	36
Issue Number	4
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-12-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunoglobulin heavy chain α-Chain Goose Molecular cloning Western-blot analysis Microbiology Biotechnology Applied Microbiology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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