NDLI: Purification of Schwann cells from adult rats by differential detachment

Content Provider	Springer Nature Link
Author	Wu, Wei Jin, Yu Qing Kretlow, James D. Xu, Liang Duan, Hui Chuan Qi, Zuo Liang
Copyright Year	2009
Abstract	Differential detachment by collagenase treatment is a new and efficient method for Schwann cell (SC) purification. As its effect on adult animals remains unclear, we have investigated the possibility of SC purification from adult rats. To avoid any systematic bias, Schwann cell purity before and after purification were compared by morphology, immunostaining of P75NTR and S100 and flow cytometric analysis. The final SC purities reached 99% as confirmed by three independent analyses SC purity and the cell yields were above 106 cells after two rounds of purification. The method of differential detachment is also suitable for SC purification in adult rats and could be useful for research and clinical applications.
Starting Page	1703
Ending Page	1708
Page Count	6
File Format	PDF
ISSN	01415492
Journal	Biotechnology Letters
Volume Number	31
Issue Number	11
e-ISSN	15736776
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-07-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Collagenase Differential detachment Nerve regeneration Purification Schwann cells Biochemistry Applied Microbiology Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Purification of Schwann cells from adult rats by differential detachment