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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhou, Bo Zhao, Xia Kawabata, Saneyuki Li, Yuhua |
| Copyright Year | 2009 |
| Abstract | We previously established a method to induce transient expression of foreign genes in intact plant tissue to detect the subcellular localization of proteins. Here, we have inserted a putative bZIP protein HY5 gene (SeqID: EU386772), isolated from the seedlings of turnips Brassica rapa L. subsp. rapa ‘Tsuda,’ and a receptor-like kinase gene AtRLK (SeqID: AY531551.1), isolated from Arabidopsis, into the plasmid pA7-GFP. We accomplished the direct incorporation of DNA into onion epidermal tissue by vacuum infiltration. By detecting GFP, which was fused with AtRLK or putative BrHY5, we determined that BrHY5 is located in the nucleus and AtRLK is located in the plasma membrane. This approach can be thus used to study the transient expression of foreign genes in intact tissue. |
| Starting Page | 1811 |
| Ending Page | 1815 |
| Page Count | 5 |
| File Format | |
| ISSN | 01415492 |
| Journal | Biotechnology Letters |
| Volume Number | 31 |
| Issue Number | 11 |
| e-ISSN | 15736776 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2009-07-18 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Brassica rapa GFP Subcellular location Transient expression Vacuum infiltration Biochemistry Applied Microbiology Biotechnology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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