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| Content Provider | Springer Nature Link |
|---|---|
| Author | Masroori, Nasser Halabian, Raheleh Mohammadipour, Mahshid Roushandeh, Amaneh Mohammadi Rouhbakhsh, Mahdi Najafabadi, Ali Jahanian Fathabad, Mahdi Edalati Salimi, Mohamad Shokrgozar, Mohamad Ali Roudkenar, Mehryar Habibi |
| Copyright Year | 2010 |
| Abstract | Recombinant coagulation factor VII (FVII) is used as a potential therapeutic intervention in hemophilia patients who produce antibodies against the coagulation factors. Mammalian cell lines provide low levels of expression, however, the Spodoptera frugiperda Sf9 cell line and baculovirus expression system are powerful systems for high-level expression of recombinant proteins, but due to the lack of endogenous vitamin K-dependent carboxylase, expression of functional FVII using this system is impossible. In the present study, we report a simple but versatile method to overcome the defect for high-level expression of the functional recombinant coagulation FVII in Sf9 cells. This method involves simultaneous expression of both human γ-carboxylase (hGC) and human FVII genes in the host. It may be possible to express other vitamin K-dependent coagulation factors using this method in the future. |
| Starting Page | 803 |
| Ending Page | 809 |
| Page Count | 7 |
| File Format | |
| ISSN | 01415492 |
| Journal | Biotechnology Letters |
| Volume Number | 32 |
| Issue Number | 6 |
| e-ISSN | 15736776 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2010-03-07 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Baculovirus γ-carboxylase Coagulation FVII Factor VII Insect cell Biochemistry Applied Microbiology Biotechnology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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