NDLI: Isolation and Characterization of the Cetn1 Gene from Tufted Deer (Elaphodus cephalophus)

Content Provider	Springer Nature Link
Author	Zhang, Wen Cao, Xiangrong Shen, Quan Liu, Wei Ni, Siyang Hua, Xiuguo Zhang, Xiran
Copyright Year	2008
Abstract	The tufted deer, a species found only in China, has polymorphic sex chromosomes (2n = 46, 47, 48). Centrins are centrosome components in species from yeast to humans. Here, the Cetn1 gene was isolated from the testis cDNA library of tufted deer, and its expression pattern, number of gene copies, and gene structure were studied. To date, for unknown reasons, satisfactory genomic DNA of the tufted deer has not been obtained. In the present study, we extracted high molecular weight genomic DNA from tissues of the tufted deer. Results showed that genomic DNA was isolated successfully and could be used for Southern blotting of the Cetn1 gene. Centrin 1 transcripts were shown to exist in testis and the retina. It has no intron and only a single copy in the genome. Protein sequence analyses indicate that the C-terminal Ser-170, which was shown to be phosphorylated in human centrin, is not conserved except in sequences from the human, chimpanzee, and tufted deer.
Starting Page	652
Ending Page	662
Page Count	11
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	46
Issue Number	9-10
e-ISSN	15734927
Language	English
Publisher	Springer US
Publisher Date	2008-08-27
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cDNA library Genomic DNA Cetn1 Southern blot Medical Microbiology Zoology Biochemistry Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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