NDLI: Mice with the Enhanced Green Fluorescent Protein Gene Knocked in to Chromosome 11 Exhibit Normal Transmission Ratios

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Mice with the Enhanced Green Fluorescent Protein Gene Knocked in to Chromosome 11 Exhibit Normal Transmission Ratios

Biochemical Genetics : Volume 43, Issue 3-4, April 2005

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Biochemical Genetics : Volume 41

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Mice with the Enhanced Green Fluorescent Protein Gene Knocked in to Chromosome 11 Exhibit Normal Transmission Ratios

Content Provider	Springer Nature Link
Author	Klysik, Jan Singer, Jeffrey D.
Copyright Year	2005
Abstract	Meiotic recombination between homologous chromosomes can be suppressed within a chosen segment by a regional inversion. In mice, this feature can be engineered and conveniently used in genetic screens to maintain chemically induced mutations within the homologous chromosome. The efficiency of an inversion-based mutagenesis screen can be substantially enhanced provided that the inversion chromosome and its wild-type (WT) homologue are both visibly tagged by two different coat color markers. Dual tagging eliminates labor associated with molecular genotyping. Previously, we reported the generation of the In(11)10Brd strain of mice carrying K14-agouti tagging a 30-cM inversion between the Trp53 and Egfr loci on mouse chromosome 11. Since K14-agouti causes yellowing of ears and tails, the In(11)10Brd mice are easily distinguishable from their WT littermates. In this paper, we describe the construction of a second strain of mice that carry the enhanced green fluorescent protein (EGFP) transgene at the Egfr locus. The EGFP carriers are visually recognizable by emitting green fluorescent light upon UV illumination. We found that the EGFP function was transmitted from one generation to another with expected Mendelian frequencies, and no detrimental effects of EGFP expression were detected in hemizygous or homozygous animals. The EGFP mice together with the previously generated In(11)10Brd inversion carriers constitute a complete set of reagents required for initiation of a regional ENU mutagenesis screen to address functionally more than one-third of mouse chromosome 11.
Starting Page	321
Ending Page	333
Page Count	13
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	43
Issue Number	5-6
e-ISSN	15734927
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2005-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	enhanced green fluorescent protein epidermal growth factor receptor mouse chromosome 11 knock-in mouse ENU mutagenesis Human Genetics Medical Microbiology Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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Mice with the Enhanced Green Fluorescent Protein Gene Knocked in to Chromosome 11 Exhibit Normal Transmission Ratios

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