NDLI: Molecular Cloning and Characterization of Mouse Testis Poly(A) Binding Protein II Encoded by the Pabp3 Gene, Which Transcomplements Meiotic Mutant sme2 of S. pombe

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Molecular Cloning and Characterization of Mouse Testis Poly(A) Binding Protein II Encoded by the Pabp3 Gene, Which Transcomplements Meiotic Mutant sme2 of S. pombe

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Molecular Cloning and Characterization of Mouse Testis Poly(A) Binding Protein II Encoded by the Pabp3 Gene, Which Transcomplements Meiotic Mutant sme2 of S. pombe

Content Provider	Springer Nature Link
Author	Okamura, Tadashi Imai, Yoshiyuki Kon, Yasuhiro Goto, Masuo Yamamoto, Masayuki Watanabe, Tomomasa
Copyright Year	2000
Abstract	A cDNA clone from a mouse testis cDNA library was isolated by the transcomplementation method using a Schizosaccharomyces pombe meiotic mutant (sme2) that is defective in meiosis I. The cDNA clone isolated has an open reading frame encoding 302 amino acids constituting a protein with a strong similarity to mouse poly(A) binding protein II (mPABII) and bovine poly(A) binding protein II (PABII). PABII is known to bind to the growing poly(A) tail and stimulates poly(A) polymerase, which catalyzes the polymerization of the mRNA poly(A) tail. Northern blot analysis of the cDNA clone identified as mPABII revealed a single transcript of 1.2 kb. This was detectable exclusively in adult testis. Immunohistochemical analysis using a polyclonal antibody demonstrated that mPABII protein was expressed in the nucleus at specific stages from late pachytene spermatocytes to round spermatids. Genetic mapping showed the Pabp3 gene encoding mPABII to be located near position 19.5 on mouse chromosome 14. These results suggest that mPABII might be involved in specific spermatogenetic cell differentiation.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	38
Issue Number	1-2
e-ISSN	15734927
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2000-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Medical Microbiology Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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Molecular Cloning and Characterization of Mouse Testis Poly(A) Binding Protein II Encoded by the Pabp3 Gene, Which Transcomplements Meiotic Mutant sme2 of S. pombe

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Molecular Cloning and Characterization of Mouse Testis Poly(A) Binding Protein II Encoded by the Pabp3 Gene, Which Transcomplements Meiotic Mutant sme2 of S. pombe