NDLI: Cloning, Heterologous Expression, and Functional Characterization of a Chitinase Gene, Lbchi32, from Limonium bicolor

Content Provider	Springer Nature Link
Author	Liu, Zhi Hua Yang, Chuan Ping Qi, Xiao Tian Xiu, Li Li Wang, Yu Cheng
Copyright Year	2010
Abstract	In the present study, an endochitinase gene, Lbchi32, was cloned from Limonium bicolor. The cDNA sequence of Lbchi32 was 1,443 bp in length and encoded 319 amino acid residues. The DNA sequence of Lbchi32 was 2,512 bp in length and contained three exons and two introns. The Lbchi32 gene was inserted into a pPIC9 vector and transferred into Pichia pastoris strains GS115 and KM71 for heterologous expression. SDS-PAGE analyses indicated that LbCHI32 was expressed in both GS115 and KM71 and that it was secreted extracellularly. The optimal reaction conditions for LbCHI32 activity are 45°C, pH 5.0, and 5 mM Ba2+. The LbCHI32 enzyme can efficiently degrade chitin, chitin derivatives, and the cell walls of different pathogenic fungi, including phytopathogenic Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Valsa sordida, Septoria tritici, and Phytophthora sojae. These findings suggest that Lbchi32 has potential use in the degradation of chitin and chitin derivatives.
Starting Page	669
Ending Page	679
Page Count	11
File Format	PDF
ISSN	00062928
Journal	Biochemical Genetics
Volume Number	48
Issue Number	7-8
e-ISSN	15734927
Language	English
Publisher	Springer US
Publisher Date	2010-05-30
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chitinase Gene cloning Heterologous expression Limonium bicolor Medical Microbiology Zoology Biochemistry Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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