NDLI: Intra-individual variability of ITS regions in entomopathogenic nematodes (Steinernematidae: Nematoda): implications for their taxonomy

Content Provider	Springer Nature Link
Author	Půža, Vladimír Chundelová, Daniela Nermuť, Jiří Žurovcová, Martina Mráček, Zdeněk
Copyright Year	2015
Abstract	The internal transcribed spacers of rDNA are among the most important molecular markers used in the taxonomy and phylogeny of entomopathogenic nematodes. However, their ease of use and the results they provide can be confounded by the presence of intra-individual variability. In the present study we screened 40 strains of S. feltiae and S. kraussei and nine strains from the “glaseri” group. In two selected strains of S. feltiae, and one strain each of S. kraussei and S. glaseri, PCR products of four virgin females were cloned, and five clones per individual female were sequenced and analyzed. In S. feltiae, intra-individual variability was compared with intraspecific variability. We revealed a high frequency of intra-individual variability, which reached 65 % in S. feltiae, 30 % in S. kraussei, and almost 90 % in the “glaseri” group. Analysis of the cloned products showed intra-individual variability in each of the tested strains and individuals. Maximum within-individual variability was over 1 % in S. feltiae and S. kraussei, and in S. glaseri reached up to 4 %. In S. feltiae, intra-individual variability equaled the variability among S. feltiae strains from different parts of the world. Our data show that intra-individual variability in the ITS regions is quite frequent in entomopathogenic nematodes. The implications of this fact to the group taxonomy are discussed.
Starting Page	547
Ending Page	554
Page Count	8
File Format	PDF
ISSN	13866141
Journal	BioControl
Volume Number	60
Issue Number	4
e-ISSN	15738248
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-02-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Steinernema Genetic diversity Intra-individual variability ITS Taxonomy Entomology Plant Pathology Agriculture Animal Ecology Animal Biochemistry Behavioral Sciences
Content Type	Text
Resource Type	Article
Subject	Insect Science Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Ecological characterisation of Steinernema siamkayai (Rhabditida: Steinernematidae), a warm-adapted entomopathogenic nematode isolate from India

Local host-dependent persistence of the entomopathogenic nematode Steinernema carpocapsae used to control the large pine weevil Hylobius abietis

Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

Efficacy of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) against the striped flea beetle, Phyllotreta striolata

Efficacy of Steinernema feltiae against the leek moth Acrolepiopsis assectella in laboratory and field conditions

Enhanced alginate capsule properties as a formulation of entomopathogenic nematodes

Steinernema sandneri n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Poland.

Control of the flat-headed root borer Capnodis tenebrionis (Linné) (Coleoptera: Buprestidae) with the entomopathogenic nematode Steinernema carpocapsae (Weiser) (Nematoda: Steinernematidae) in a chitosan formulation in apricot orchards

Immune defenses of Agriotes lineatus larvae against entomopathogenic nematodes

Intra-individual variability of ITS regions in entomopathogenic nematodes (Steinernematidae: Nematoda): implications for their taxonomy

Similar Documents

Ecological characterisation of Steinernema siamkayai (Rhabditida: Steinernematidae), a warm-adapted entomopathogenic nematode isolate from India

Local host-dependent persistence of the entomopathogenic nematode Steinernema carpocapsae used to control the large pine weevil Hylobius abietis

Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

Efficacy of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) against the striped flea beetle, Phyllotreta striolata

Efficacy of Steinernema feltiae against the leek moth Acrolepiopsis assectella in laboratory and field conditions

Enhanced alginate capsule properties as a formulation of entomopathogenic nematodes

Steinernema sandneri n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Poland.

Control of the flat-headed root borer Capnodis tenebrionis (Linné) (Coleoptera: Buprestidae) with the entomopathogenic nematode Steinernema carpocapsae (Weiser) (Nematoda: Steinernematidae) in a chitosan formulation in apricot orchards

Immune defenses of Agriotes lineatus larvae against entomopathogenic nematodes

Intra-individual variability of ITS regions in entomopathogenic nematodes (Steinernematidae: Nematoda): implications for their taxonomy