NDLI: Methodological models for in vitro amplification and maintenance of human articular chondrocytes from elderly patients

Content Provider	Springer Nature Link
Author	Carossi, Anna Maria Recenti, Raffaella Carossi, Roberto Piscitelli, Elisabetta Gozzini, Alessia Martineti, Valentina Mavilia, Carmelo Franchi, Alessandro Danielli, Daniele Aglietti, Paolo Ciardullo, Antonio Galli, Gianna Tognarini, Isabella Moggi Pigne, Alberto Cagni, Mario Brandi, Maria Luisa
Copyright Year	2007
Abstract	Articular cartilage defects, an exceedingly common problem closely correlated with advancing age, is characterized by lack of spontaneous resolution because of the limited regenerative capacity of adult articular chondrocytes. Medical and surgical therapies yield unsatisfactory short-lasting results. Recently, cultured autologous chondrocytes have been proposed as a source to promote repair of deep cartilage defects. Despite encouraging preliminary results, this approach is not yet routinely applicable in clinical practice, but for young patients. One critical points is the isolation and ex vivo expansion of large enough number of differentiated articular chondrocytes. In general, human articular chondrocytes grown in monolayer cultures tend to undergo dedifferentiation. This reversible process produces morphological changes by which cells acquire fibroblast-like features, loosing typical functional characteristics, such as the ability to synthesize type II collagen. The aim of this study was to isolate human articular chondrocytes from elderly patients and to carefully characterize their morphological, proliferative, and differentiative features. Cells were morphologically analyzed by optic and transmission electron microscopy (TEM). Production of periodic acid-schiff (PAS)-positive cellular products and of type II collagen mRNA was monitored at different cellular passages. Typical chondrocytic characteristics were also studied in a suspension culture system with cells encapsulated in alginate-polylysine-alginate (APA) membranes. Results showed that human articular chondrocytes can be expanded in monolayers for several passages, and then microencapsulated, retaining their morphological and functional characteristics. The results obtained could contribute to optimize expansion and redifferentiation sequences for applying cartilage tissue engineering in the elderly patients.
Starting Page	483
Ending Page	498
Page Count	16
File Format	PDF
ISSN	13895729
Journal	Biogerontology
Volume Number	8
Issue Number	5
e-ISSN	15736768
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-03-20
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human articular chondrocytes Type I collagen Type II collagen Alginate Cell therapy Developmental Biology Geriatrics/Gerontology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Aging Gerontology Geriatrics and Gerontology

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