NDLI: Possible role of subunit A of nuclearfactor Y (NF-YA) in normal human diploidfibroblasts during senescence

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Possible role of subunit A of nuclearfactor Y (NF-YA) in normal human diploidfibroblasts during senescence

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Biogerontology : Volume 1, Issue 2, June 2000

Biogerontology : Volume 1, Issue 1, March 2000

Possible role of subunit A of nuclearfactor Y (NF-YA) in normal human diploidfibroblasts during senescence

Content Provider	Springer Nature Link
Author	Matuoka, Koozi Chen, Kuang Yu
Copyright Year	2000
Abstract	NF-Y, a heterotrimeric CCAAT binding protein, may have a role in regulating some G1/S genes whose expressions are attenuated during replicative senescence [Matuoka and Chen (1999) Exp Cell Res 253: 365–371]. The hallmark of replicative senescence is the loss of dividing potential. Hence, attenuation of G1/S gene expressions may be causally related to aging. To understand how NF-Y is involved in regulating G1/S genes during replicative senescence, we have examined the expressions of three NF-Y subunit genes in human IMR-90 cells over the entire course of their life-span. The mRNA levels of NF-YA, B, and C did not show any age-dependent change. In contrast, the protein level of NF-YA exhibited a significant and progressive decrease during cell senescence. Cross-linking experiments indicated that NF-Y may interact with proteins such as GCN5 and P/CAF. Co-transfection of cells with plasmid encoding NF-YA protein enhanced the expression of reporter gene fused with G1/S gene promoter that contains NF-Y sites. In contrast, co-transfection with plasmid encoding the dominant negative NF-YA mutant suppressed the expression of the reporter genes. Transient transfection of human cells with dominant negative NF-YA mutant could lead to an increase in neutral β-galactosidase activity, a marker of cell senescence. These results support the view that NF-Y may have a role in cell senescence.
Starting Page	261
Ending Page	271
Page Count	11
File Format	PDF
ISSN	13895729
Journal	Biogerontology
Volume Number	1
Issue Number	3
e-ISSN	15736768
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2000-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Geriatrics/Gerontology Proteomics Cell Biology Developmental Biology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Aging Gerontology Geriatrics and Gerontology

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