NDLI: Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

Content Provider	Springer Nature Link
Author	Romava, O. A. Grigor’ev, T. E. Goncharov, M. E. Rudyak, S. G. Solov’yova, E. V. Krasheninnikov, S. T. Saprykin, V. P. Sytina, E. V. Chvalun, S. N. Pal’tsev, M. A. Panteleev, A. A.
Copyright Year	2015
Abstract	We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers. The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes). In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix. However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds. The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment. Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective. We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.
Starting Page	557
Ending Page	566
Page Count	10
File Format	PDF
ISSN	00074888
Journal	Bulletin of Experimental Biology and Medicine
Volume Number	159
Issue Number	4
e-ISSN	15738221
Language	English
Publisher	Springer US
Publisher Date	2015-09-22
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	keratinocytes fibroblasts biocompatibility skin equivalent Biomedicine general Internal Medicine Cell Biology Pathology Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology

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