NDLI: Functional analysis of a chitinase gene during the larval-nymph transition in Panonychus citri by RNA interference

Content Provider	Springer Nature Link
Author	Xia, Wen Kai Shen, Xiao Min Ding, Tian Bo Niu, Jin Zhi Zhong, Rui Liao, Chong Yu Feng, Ying Cai Dou, Wei Wang, Jin Jun
Copyright Year	2016
Abstract	Chitinases are hydrolytic enzymes that are required for chitin degradation and reconstruction in arthropods. In this study, we report a cDNA sequence encoding a putative chitinase (PcCht1) from the citrus red mite, Panonychus citri. The PcCht1 (564 aa) possessed a signal peptide, a conserver domain, and a chitin-binding domain. Structural and phylogenetic analyses found that PcCht1 had high sequence similarity to chitinases in Tetranychus urticae. Real-time quantitative PCR analyses showed that the transcript levels of PcCht1 peaked periodically in larval and nymph stages. Moreover, significant increase of PcCht1 transcript level in the larvae was observed upon the exposure of diflubenzuron. In contrast, exposures of the larvae to diflubenzuron resulted in the decreased chitin content. Furthermore, through a feeding-based RNA interference approach, we were able to reduce the PcCht1 transcript level by 59.7 % in the larvae, and consequently the treated larvae showed a very low molting rate compared with the control. Our results expanded the understanding of the important role of PcCht1 in the growth and development of P. citri.
Starting Page	1
Ending Page	15
Page Count	15
File Format	PDF
ISSN	01688162
Journal	Experimental & Applied Acarology
Volume Number	70
Issue Number	1
e-ISSN	15729702
Language	English
Publisher	Springer International Publishing
Publisher Date	2016-07-07
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Panonychus citri Citrus red mite Chitinase Diflubenzuron RNA interference Entomology Animal Systematics/Taxonomy/Biogeography Animal Genetics and Genomics Animal Ecology Life Sciences
Content Type	Text
Resource Type	Article
Subject	Ecology Medicine Insect Science

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Functional analysis of a chitinase gene during the larval-nymph transition in Panonychus citri by RNA interference

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