NDLI: A novel killer protein from Pichia kluyveri isolated from an Algerian soil: purification and characterization of its in vitro activity against food and beverage spoilage yeasts

Content Provider	Springer Nature Link
Author	Labbani, Fatima Zohra Kenza Turchetti, Benedetta Bennamoun, Leila Dakhmouche, Scheherazad Roberti, Rita Corazzi, Lanfranco Meraihi, Zahia Buzzini, Pietro
Copyright Year	2015
Abstract	A novel killer protein (Pkkp) secreted by a Pichia kluyveri strain isolated from an Algerian soil was active against food and beverage spoilage yeasts of the genera Dekkera, Kluyveromyces, Pichia, Saccharomyces, Torulaspora, Wickerhamomyces and Zygosaccharomyces. After purification by gel filtration chromatography Pkkp revealed an apparent molecular mass of 54 kDa with SDS-PAGE. Minimum inhibitory concentrations (MICs) of purified Pkkp exhibited a high in vitro activity against Dekkera bruxellensis (MICs from 64,000- to 256,000-fold lower than that exhibited by potassium metabisulphite) and Saccharomyces cerevisiae (MICs from 32,000- to 64,000- fold lower than potassium sorbate). No in vitro synergistic interactions (calculated by FIC index − Σ FIC) were observed when Pkkp was used in combination with potassium metabisulphite, potassium sorbate, or ethanol. Pkkp exhibited a dose–response effect against D. bruxellensis and S. cerevisiae in a low-alcoholic drink and fruit juice, respectively. The results of the present study suggest that Pkkp could be proposed as a novel food-grade compound useful for the control of food and beverage spoilage yeasts.
Starting Page	961
Ending Page	970
Page Count	10
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	107
Issue Number	4
e-ISSN	15729699
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-01-25
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Yeast killer protein Pichia kluyveri Susceptibility testing Spoilage yeasts Minimum inhibitory concentrations (MICs) Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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