NDLI: Steady state growth space study of Lactococcus lactis in D-stat cultures

Content Provider	Springer Nature Link
Author	Lahtvee, Petri Jaan Valgepea, Kaspar Nahku, Ran Abner, Kristo Adamberg, Kaarel Vilu, Raivo
Copyright Year	2009
Abstract	Growth space of Lactococcus lactis subsp. lactis IL1403 was studied at constant growth rate using D-stat cultivation technique. Starting from steady state conditions in a chemostat culture (μ = 0.2 h−1), the pH and/or temperature were continuously changed in the range of 5.4–6.4 and 26–34°C, respectively, followed by the return to the initial environmental conditions. Based on substrate consumption and product formation yields and expression changes of 1,920 genes, it was shown that changes of physiological state were not dependent on the direction of movement (from pH 6.3 to 5.4 or from 5.4 to 6.3), showing that quasi steady state values in D-stat corresponded to the steady state values in chemostats. Relative standard deviation of growth characteristics in triplicate D-stat experiments was below 10%. Continuing the experiment and reestablishing initial growth conditions revealed in average 7% difference (hysteresis) in growth characteristics when comparing chemostat steady state cultures prior and after the change of environmental conditions. Similarly, shifts were also seen at gene expression levels. The large amount of quantitatively reliable data obtained in this study provided a new insight into dynamic properties of bacterial physiology, and can be used for describing the growth space of microorganisms by modeling cell metabolism.
Starting Page	487
Ending Page	496
Page Count	10
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	96
Issue Number	4
e-ISSN	15729699
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-07-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Continuous culture Lactococcus lactis Acid stress Growth space Transcriptome Medical Microbiology Soil Science & Conservation Plant Sciences Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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