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| Content Provider | Springer Nature Link |
|---|---|
| Author | Silva Jiménez, Hortencia Zazueta voa, Vanesa Durón Castellas, Arelí Rodríguez Robelo, Carmen Leal Morales, Carlos A. Zazueta Sandoval, Roberto |
| Copyright Year | 2009 |
| Abstract | In previous studies, Mucor circinelloides YR-1 isolated from petroleum-contaminated soils grown in decane as sole carbon source, showed fatty alcohol oxidase (FAO) activities in either particulate or soluble fractions from a cell-free extract. One is associated to internal membranes (mFAO) and the other one is soluble (sFAO). Both activities appear to be located in the cells in specific compartments other than peroxisomes. Results suggested that mFAO could be located on the inner face of the membrane of these compartments, and sFAO could be in the lumen of the specific compartments. This study reports on the intracellular distribution of FAO activity and the purification of sFAOs and mFAO after several different procedures for release from the membranous fraction using the mixed membrane fraction (MMF) after cellular homogenization as enzymatic source. Results with the purified mFAO show, by molecular weight criteria, that the enzyme has only one type of subunit with molecular mass of 46 kDa, with two isoelectric point components: 6.0 and 6.3. We found that mFAO is strongly associated to the MMF, possibly in a transitory fashion. Using non-denaturating gels, we suggest that sFAO and mFAO have the same subunits in their native structures, and, due to their native molecular weight of approximately 350 kDa, each could be natively structured as an octameric complex. |
| Starting Page | 527 |
| Ending Page | 535 |
| Page Count | 9 |
| File Format | |
| ISSN | 00036072 |
| Journal | Antonie van Leeuwenhoek |
| Volume Number | 96 |
| Issue Number | 4 |
| e-ISSN | 15729699 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2009-07-30 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Hydrocarbon biodegradation Aliphatic hydrocarbons Filamentous fungi Medical Microbiology Soil Science & Conservation Plant Sciences Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Microbiology |
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