NDLI: Contribution of the thermotolerance genomic island to increased thermal tolerance in Cronobacter strains

Content Provider	Springer Nature Link
Author	Orieskova, Maria Kajsik, Michal Szemes, Tomas Holy, Ondrej Forsythe, Stephen Turna, Jan Drahovska, Hana
Copyright Year	2016
Abstract	Cronobacter spp. are opportunistic pathogens associated with serious infections in neonates. Increased stress tolerance, including the thermotolerance of some Cronobacter strains, can promote their survival in production facilities and thus raise the possibility of contamination of dried infant formula which has been identified as a potential source of infection. Some Cronobacter strains contain a genomic island, which might be responsible for increased thermotolerance. By analysis of Cronobacter sequenced genomes this determinant was found to be present in only 49/73 Cronobacter sakazakii strains and in 9/14 Cronobacter malonaticus strains. The island was also found in 16/17 clinical isolates originating from two hospitals. Two configurations of the locus were detected; the first one with the size of 18 kbp containing the thrB-Q genes and a shorter version (6 kbp) harbouring only the thrBCD and thrOP genes. Strains containing the thermotolerance island survived significantly better at 58 °C comparing to a C. sakazakii isogenic mutant lacking the island and strains with the longer version of the island were 2–10 times more tolerant than those with the shortened sequence. The function of the genomic island was further confirmed by its cloning into a low-copy vector and transforming it into the isogenic mutant. Different levels of rpoS, encoding for stress-response sigma factor, expression were also associated with variability in strain thermotolerance.
Starting Page	405
Ending Page	414
Page Count	10
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	109
Issue Number	3
e-ISSN	15729699
Language	English
Publisher	Springer International Publishing
Publisher Date	2016-01-09
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cronobacter spp. Heat stress rpoS Thermotolerance Thermotolerance island Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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Contribution of the thermotolerance genomic island to increased thermal tolerance in Cronobacter strains.

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