NDLI: Mutagenesis of nisin’s leader peptide proline strongly modulates export of precursor nisin

Content Provider	Springer Nature Link
Author	Plat, Annechien Kuipers, Anneke Crabb, Joe Rink, Rick Moll, Gert N.
Copyright Year	2016
Abstract	The lantibiotic nisin is produced by Lactococcus lactis as a precursor peptide comprising a 23 amino acid leader peptide and a 34 amino acid post-translationally modifiable core peptide. We previously demonstrated that the conserved FNLD part of the leader is essential for intracellular enzyme-catalyzed introduction of lanthionines in the core peptide and also for transporter-mediated export, whereas other positions are subject to large mutational freedom. We here demonstrate that, in the absence of the extracellular leader peptidase, NisP, export of precursor nisin via the modification and transporter enzymes, NisBTC, is strongly affected by multiple substitutions of the leader residue at position -2, but not by substitution of positions in the vicinity of this site. Export levels of precursor nisin increased by more than 70% for position -2 mutants Asp, Thr, Ser, Trp, Lys, Val and decreased more than 70% for Cys, His, Met. In a strain with leader peptidase, the Pro-2Lys and Pro-2Asp precursor nisins were less efficiently cleaved by NisP than wild type precursor nisin. Taken together, the wild type precursor nisin with a proline at position -2 allows balanced export and cleavage efficiencies by precursor nisin’s transporter and leader peptidase.
Starting Page	321
Ending Page	330
Page Count	10
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	110
Issue Number	3
e-ISSN	15729699
Language	English
Publisher	Springer International Publishing
Publisher Date	2016-11-10
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lactococcus lactis Lanthionine Lanthipeptide Leader peptide Nisin Transporter Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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