NDLI: Determinants of tolerance to inhibitors in hardwood spent sulfite liquor in genome shuffled Pachysolen tannophilus strains

Content Provider	Springer Nature Link
Author	Harner, Nicole K. Bajwa, Paramjit K. Formusa, Philip A. Austin, Glen D. Habash, Marc B. Trevors, Jack T. Chan, Chi Kin Ho, Chi Yip Lee, Hung
Copyright Year	2015
Abstract	Genome shuffling was used to obtain Pachysolen tannophilus mutants with improved tolerance to inhibitors in hardwood spent sulfite liquor (HW SSL). Genome shuffled strains (GHW301, GHW302 and GHW303) grew at higher concentrations of HW SSL (80 % v/v) compared to the HW SSL UV mutant (70 % v/v) and the wild-type (WT) strain (50 % v/v). In defined media containing acetic acid (0.70–0.90 % w/v), GHW301, GHW302 and GHW303 exhibited a shorter lag compared to the acetic acid UV mutant, while the WT did not grow. Genome shuffled strains produced more ethanol than the WT at higher concentrations of HW SSL and an aspen hydrolysate. To identify the genetic basis of inhibitor tolerance, whole genome sequencing was carried out on GHW301, GHW302 and GHW303 and compared to the WT strain. Sixty single nucleotide variations were identified that were common to all three genome shuffled strains. Of these, 40 were in gene sequences and 20 were within 5 bp–1 kb either up or downstream of protein encoding genes. Based on the mutated gene products, mutations were grouped into functional categories and affected a variety of cellular functions, demonstrating the complexity of inhibitor tolerance in yeast. Sequence analysis of UV mutants (UAA302 and UHW303) from which GHW301, GHW302 and GHW303 were derived, confirmed the success of our cross-mating based genome shuffling strategy. Whole-genome sequencing analysis allowed identification of potential gene targets for tolerance to inhibitors in lignocellulosic hydrolysates.
Starting Page	811
Ending Page	834
Page Count	24
File Format	PDF
ISSN	00036072
Journal	Antonie van Leeuwenhoek
Volume Number	108
Issue Number	4
e-ISSN	15729699
Language	English
Publisher	Springer International Publishing
Publisher Date	2015-08-01
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Genome sequencing Genome shuffling Inhibitors Lignocellulose Pentose-fermenting Tolerance Microbiology Medical Microbiology Plant Sciences Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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