NDLI: Genome-wide expression analysis of wounded skin reveals novel genes involved in angiogenesis

Content Provider	Springer Nature Link
Author	Brönneke, Simone Brückner, Bodo Söhle, Jörn Siegner, Ralf Smuda, Christoph Stäb, Franz Wenck, Horst Kolbe, Ludger Grönniger, Elke Winnefeld, Marc
Copyright Year	2015
Abstract	Wound healing is a multistage process involving collaborative efforts of different cell types and distinct cellular functions. Among others, the high metabolic activity at the wound site requires the formation and sprouting of new blood vessels (angiogenesis) to ensure an adequate supply of oxygen and nutrients for a successful healing process. Thus, a cutaneous wound healing model was established to identify new factors that are involved in vascular formation and remodeling in human skin after embryonic development. By analyzing global gene expression of skin biopsies obtained from wounded and unwounded skin, we identified a small set of genes that were highly significant differentially regulated in the course of wound healing. To initially investigate whether these genes might be involved in angiogenesis, we performed siRNA experiments and analyzed the knockdown phenotypes using a scratch wound assay which mimics cell migration and proliferation in vitro. The results revealed that a subset of these genes influence cell migration and proliferation in primary human endothelial cells (EC). Furthermore, histological analyses of skin biopsies showed that two of these genes, ALBIM2 and TMEM121, are colocalized with CD31, a well known EC marker. Taken together, we identified new genes involved in endothelial cell biology, which might be relevant to develop therapeutics not only for impaired wound healing but also for chronic inflammatory disorders and/or cardiovascular diseases.
Starting Page	361
Ending Page	371
Page Count	11
File Format	PDF
ISSN	09696970
Journal	Angiogenesis
Volume Number	18
Issue Number	3
e-ISSN	15737209
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-05-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Angiogenesis Genome-wide expression analyses Human skin Wound healing Cancer Research Biomedicine general Cell Biology Cardiology Ophthalmology Oncology
Content Type	Text
Resource Type	Article
Subject	Physiology Clinical Biochemistry Cancer Research

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