NDLI: Effect of Zinc and Nitric Oxide on Monocyte Adhesion to Endothelial Cells under Shear Stress

Content Provider	Springer Nature Link
Author	Lee, Sungmun Eskin, Suzanne G. Shah, Ankit K. Schildmeyer, Lisa A. McIntire, Larry V.
Copyright Year	2011
Abstract	This study describes the effect of zinc on monocyte adhesion to endothelial cells under different shear stress regimens, which may trigger atherogenesis. Human umbilical vein endothelial cells were exposed to steady shear stress (15 dynes/cm2 or 1 dyne/cm2) or reversing shear stress (time average 1 dyne/cm2) for 24 h. In all shear stress regimes, zinc deficiency enhanced THP-1 cell adhesion, while heparinase III reduced monocyte adhesion following reversing shear stress exposure. Unlike other shear stress regimes, reversing shear stress alone enhanced monocyte adhesion, which may be associated with increased H2O2 and superoxide together with relatively low levels of nitric oxide (NO) production. L-NG-Nitroarginine methyl ester (L-NAME) treatment increased monocyte adhesion under 15 dynes/cm2 and under reversing shear stress. After reversing shear stress, monocyte adhesion dramatically increased with heparinase III treatment followed by a zinc scavenger. Static culture experiments supported the reduction of monocyte adhesion by zinc following endothelial cell cytokine activation. These results suggest that endothelial cell zinc levels are important for the inhibition of monocyte adhesion to endothelial cells, and may be one of the key factors in the early stages of atherogenesis.
Starting Page	697
Ending Page	706
Page Count	10
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	40
Issue Number	3
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2011-10-19
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mechanical forces Vascular endothelial cells Heparan sulfate proteoglycans Reactive oxygen species Biochemistry Biophysics and Biological Physics Mechanics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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Effect of Zinc and Nitric Oxide on Monocyte Adhesion to Endothelial Cells under Shear Stress

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