NDLI: Endothelial Cell Layer Subjected to Impinging Flow Mimicking the Apex of an Arterial Bifurcation

Content Provider	Springer Nature Link
Author	Szymanski, Michael P. Metaxa, Eleni Meng, Hui Kolega, John
Copyright Year	2008
Abstract	Little is known about endothelial responses to the impinging flow hemodynamics that occur at arterial bifurcation apices, where intracranial aneurysms usually form. Such hemodynamic environments are characterized by high wall shear stress (WSS >40 dynes/cm2) and high wall shear stress gradients (WSSG >300 dynes/cm3). In this study, confluent bovine aortic endothelial cells were exposed to impinging flow in a T-shaped chamber designed to mimic a bifurcation. After 24–72 h under flow, cells around the stagnation point maintained polygonal shapes but cell density was reduced, whereas cells in adjacent downstream regions exposed to very high WSS and WSSG were elongated, aligned parallel to flow, and at higher density. Such behavior was not blocked by inhibiting proliferation, indicating that cells migrated downstream from the stagnation point in response to impinging flow. Furthermore, although the area of highest cell density moved downstream and away from the impingement point over time, it never moved beyond the WSS maximum. The accumulation of cells upstream of maximal WSS and downstream of maximal WSSG suggests that positive WSSG is responsible for the observed migration. These results demonstrate a unique endothelial response to aneurysm-promoting flow environments at bifurcation apices.
Starting Page	1681
Ending Page	1689
Page Count	9
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	36
Issue Number	10
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2008-07-25
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Wall shear stress Spatial wall shear stress gradient Migration Aneurysm Biochemistry Mechanics Biophysics/Biomedical Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Endothelial Cell Layer Subjected to Impinging Flow Mimicking the Apex of an Arterial Bifurcation

High Fluid Shear Stress and Spatial Shear Stress Gradients Affect Endothelial Proliferation, Survival, and Alignment

High Wall Shear Stress and Spatial Gradients in Vascular Pathology: A Review

Effects of Arterial Bifurcation Geometry on Nucleotide Concentration at the Endothelium

The Flow Field along the Entire Length of Mouse Aorta and Primary Branches

Interaction of Wall Shear Stress Magnitude and Gradient in the Prediction of Arterial Macromolecular Permeability

Effect of Cell-Free Layer Variation on Arteriolar Wall Shear Stress

Endothelial Transcriptome Profiles In Vivo in Complex Arterial Flow Fields

Influence of Pulsatile Flow on LDL Transport in the Arterial Wall

Endothelial Cell Layer Subjected to Impinging Flow Mimicking the Apex of an Arterial Bifurcation

Similar Documents

Endothelial Cell Layer Subjected to Impinging Flow Mimicking the Apex of an Arterial Bifurcation

High Fluid Shear Stress and Spatial Shear Stress Gradients Affect Endothelial Proliferation, Survival, and Alignment

High Wall Shear Stress and Spatial Gradients in Vascular Pathology: A Review

Effects of Arterial Bifurcation Geometry on Nucleotide Concentration at the Endothelium

The Flow Field along the Entire Length of Mouse Aorta and Primary Branches

Interaction of Wall Shear Stress Magnitude and Gradient in the Prediction of Arterial Macromolecular Permeability

Effect of Cell-Free Layer Variation on Arteriolar Wall Shear Stress

Endothelial Transcriptome Profiles In Vivo in Complex Arterial Flow Fields

Influence of Pulsatile Flow on LDL Transport in the Arterial Wall

Endothelial Cell Layer Subjected to Impinging Flow Mimicking the Apex of an Arterial Bifurcation