NDLI: Combined Experimental and Mathematical Approach for Development of Microfabrication-Based Cancer Migration Assay

Content Provider	Springer Nature Link
Author	Sarkar, Saheli Bustard, Bethany L. Welter, Jean F. Baskaran, Harihara
Copyright Year	2011
Abstract	Migration of cancer cells is a key determinant of metastasis, which is correlated with poor prognosis in patients. Evidence shows that cancer cell motility is regulated by stromal cell interactions. To quantify the role of homotypic and heterotypic cell–cell interaction in migration, a two-dimensional migration assay has been developed by microfabrication techniques. Two breast cancer cell lines, MDA-MB-231 and MDA-MB-453, were used to develop micropatterns of cancer cells (cell islands) that revealed distinct migration profiles in this assay. Although the individual migration rates of these cells showed only a sevenfold difference, MDA-MB-453 islands migrated significantly lower than MDA-MB-231 islands, indicating differential regulation of migration in isolated cells vs. islands. Island size had the greatest impact on migration, primarily for MDA-MB-231 cells. Migration of MDA-MB-231 islands was decreased by interaction with homotypic cells, and significantly more by heterotypic non-cancer-associated fibroblasts. In addition, a mathematical model of island migration in multi-cellular population has been developed using Stefan–Maxwell’s equation. The model showed qualitative agreement with experimental results and predicted a biphasic relation between cell densities and island sizes. The combined experimental and mathematical model can be used to quantitatively study the impact of cell–cell interactions on migration.
Starting Page	2346
Ending Page	2359
Page Count	14
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	39
Issue Number	9
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2011-06-24
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfabrication Breast cancer Stromal cells Mathematical model Multi-component transport Soft lithography Homotypic interactions Heterotypic interactions Biochemistry Biomedical Engineering Mechanics Biophysics and Biological Physics Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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