NDLI: Estimating in Vitro Mitochondrial Oxygen Consumption During Muscle Contraction and Recovery: A Novel Approach that Accounts for Diffusion

Content Provider	Springer Nature Link
Author	Dash, Ranjan K. Bell, Bradley M. Kushmerick, Martin J. Vicini, Paolo
Copyright Year	2005
Abstract	A deconvolution algorithm, based on a Bayesian statistical framework and smoothing spline technique, is applied to reconstructing input functions from noisy measurements in biological systems. Deconvolution is usually ill-posed. However, placing a Bayesian prior distribution on the input function can make the problem well-posed. Using this algorithm and a computational model of diffusional oxygen transport in an approximately cylindrical muscle (about 0.5-mm diameter and 10-mm long mouse leg muscle), the time course of muscle oxygen uptake and mitochondrial oxygen consumption, both during isometric twitch contractions (at various frequencies) and the recovery period, is estimated from polarographic measurements of oxygen concentration on the muscle surface. An important feature of our experimental protocol is the availability of data for the apparatus characteristics. From these time courses, the actual mitochondrial consumption rates during resting and exercise states can be estimated. Mitochondrial oxygen consumption rate increased during stimulation to a maximum steady state value approximately five times of the resting value of 0.63 nmol/s/g wet weight for the stimulation conditions studied. Diffusion slowed the kinetic responses to the contraction but not the steady state fluxes during the stimulation interval.
Starting Page	343
Ending Page	355
Page Count	13
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	33
Issue Number	3
e-ISSN	15739686
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2005-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Muscle contraction Muscle oxygen uptake Mitochondrial oxygen consumption Muscle energetics Oxygen diffusion Deconvolution Biochemistry Mechanics Biophysics/Biomedical Physics Vibration, Dynamical Systems, Control Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Evaluation of a Deformable Musculoskeletal Model for Estimating Muscle–Tendon Lengths During Crouch Gait

Importance of Temperature and Humidity in the Measurement of Pulmonary Oxygen Uptake per Breath During Anesthesia

Modeling Advection and Diffusion of Oxygen in Complex Vascular Networks

Handling Non-Negativity in Deconvolution of Physiological Signals: A Nonlinear Stochastic Approach

Linking Pulmonary Oxygen Uptake, Muscle Oxygen Utilization and Cellular Metabolism during Exercise

Genetics of Energetics: Transcriptional Responses in Cardiac Metabolism

Imaging Epicardial Oxygen

Site-Specific Molecular Diffusion in Articular Cartilage Measured using Fluorescence Recovery after Photobleaching

Wavelet Entropy for Subband Segmentation of EEG During Injury and Recovery

Estimating in Vitro Mitochondrial Oxygen Consumption During Muscle Contraction and Recovery: A Novel Approach that Accounts for Diffusion

Similar Documents

Evaluation of a Deformable Musculoskeletal Model for Estimating Muscle–Tendon Lengths During Crouch Gait

Importance of Temperature and Humidity in the Measurement of Pulmonary Oxygen Uptake per Breath During Anesthesia

Modeling Advection and Diffusion of Oxygen in Complex Vascular Networks

Handling Non-Negativity in Deconvolution of Physiological Signals: A Nonlinear Stochastic Approach

Linking Pulmonary Oxygen Uptake, Muscle Oxygen Utilization and Cellular Metabolism during Exercise

Genetics of Energetics: Transcriptional Responses in Cardiac Metabolism

Imaging Epicardial Oxygen

Site-Specific Molecular Diffusion in Articular Cartilage Measured using Fluorescence Recovery after Photobleaching

Wavelet Entropy for Subband Segmentation of EEG During Injury and Recovery

Estimating in Vitro Mitochondrial Oxygen Consumption During Muscle Contraction and Recovery: A Novel Approach that Accounts for Diffusion