NDLI: Altered Flow Changes Thrombin Generation Rate of Circulating Platelets

Content Provider	Springer Nature Link
Author	Yin, Wei Bond, Kyle Rouf, Farzana Rubenstein, David A.
Copyright Year	2015
Abstract	Shear stress affects platelet participation in coagulation. Many numerical models have been developed to describe coagulation kinetics. However, most of those models used rate constants determined under static conditions. Little is known about the effects of flow on coagulation rate constants. In the present study, platelets were exposed to constant or pulsatile shear stress/rate, with or without prothrombin, factor Xa, and factor Va. Thrombin generation was measured using a modified prothrombinase assay, and the overall thrombin generation rate was solved using typical Michaelis–Menten kinetics. Platelet surface P-selectin and phosphatidylserine (PS) expression was measured using flow cytometry. The results demonstrated that the concentration of factor Va had a dominant effect on thrombin generation rate under flow. In comparison, the expression of PS was less sensitive to altered flow. The lumped overall rate constant for prothrombin conversion to thrombin was significantly affected by the shear forces that were applied to the coagulation complex. Constant shear stress/rate induced faster thrombin generation compared to pulsatile shear stress/rate, but elevated shear stress/rate did not necessarily enhance thrombin generation. Therefore, the overall thrombin generation rate is dynamic and must be described as a function of shear stress/rate, shear exposure time and the immediate availability of coagulation proteins.
Starting Page	2827
Ending Page	2837
Page Count	11
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	43
Issue Number	12
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2015-06-03
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Platelets Thrombin Kinetics Rate constants Shear stress/rate Biomedicine general Biomedical Engineering Biophysics and Biological Physics Mechanics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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