NDLI: Design Considerations for a Microfluidic Device to Quantify the Platelet Adhesion to Collagen at Physiological Shear Rates

Content Provider	Springer Nature Link
Author	Sarvepalli, Durga P. Schmidtke, David W. llert, Matthias U.
Copyright Year	2009
Abstract	Accurate assessment of blood platelet function is essential in understanding thrombus formation which plays a central role in cardiovascular disease. Parallel plate flow chambers have been widely used as they allow for platelet adhesion on a collagen surface at physiologically relevant fluid mechanical forces. Standard parallel plate flow chambers typically need several milliliters of blood, which is substantially more than can be obtained from small animals. We designed, fabricated, and assessed the functionality of a microfluidic channel with a width of 500 μm and a height of 50 μm in which a wall shear rate of 1000 s−1 can be achieved with a flow rate of 15 μL/min. The velocity distribution in the microchannel predicted from the equations of motion was compared to experimentally measured velocities of fluorescent beads. This analysis showed that the motion of beads was quite similar to the predicted motion. Adhesion of platelets from whole blood at a shear rate of 1000 s−1 onto a collagen surface using the microfluidic flow channel was qualitatively similar to platelet adhesion observed with a standard sized parallel plate flow chamber. After 5 min flow the surface coverage of platelets in the microfluidic device was about 55% while in a traditional size flow chamber the surface coverage was about 75%. This suggests that the microfluidic flow chamber can be used to quantify platelet adhesion for system where only very small amounts of blood are available.
Starting Page	1331
Ending Page	1341
Page Count	11
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	37
Issue Number	7
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2009-05-08
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidics Thrombosis Platelets Shear stress Biochemistry Mechanics Biophysics/Biomedical Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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