NDLI: RETRACTED ARTICLE: Non-thermal Plasma Induces Apoptosis in Melanoma Cells via Production of Intracellular Reactive Oxygen Species

Content Provider	Springer Nature Link
Author	Sensenig, Rachel Kalghatgi, Sameer Cerchar, Ekaterina Fridman, Gregory Shereshevsky, Alexey Torabi, Behzad Arjunan, Krishna Priya Podolsky, Erica Fridman, Alexander Friedman, Gary Azizkhan Clifford, Jane Brooks, Ari D.
Copyright Year	2010
Abstract	Non-thermal atmospheric pressure dielectric barrier discharge (DBD) plasma may provide a novel approach to treat malignancies via induction of apoptosis. The purpose of this study was to evaluate the potential of DBD plasma to induce apoptosis in melanoma cells. Melanoma cells were exposed to plasma at doses that did not induce necrosis, and cell viability and apoptotic activity were evaluated by Trypan blue exclusion test, Annexin-V/PI staining, caspase-3 cleavage, and TUNEL® analysis. Trypan blue staining revealed that non-thermal plasma treatment significantly decreased the viability of cells in a dose-dependent manner 3 and 24 h after plasma treatment. Annexin-V/PI staining revealed a significant increase in apoptosis in plasma-treated cells at 24, 48, and 72 h post-treatment (p < 0.001). Caspase-3 cleavage was observed 48 h post-plasma treatment at a dose of 15 J/cm2. TUNEL® analysis of plasma-treated cells demonstrated an increase in apoptosis at 48 and 72 h post-treatment (p < 0.001) at a dose of 15 J/cm2. Pre-treatment with N-acetyl-l-cysteine (NAC), an intracellular reactive oxygen species (ROS) scavenger, significantly decreased apoptosis in plasma-treated cells at 5 and 15 J/cm2. Plasma treatment induces apoptosis in melanoma cells through a pathway that appears to be dependent on production of intracellular ROS. DBD plasma production of intracellular ROS leads to dose-dependent DNA damage in melanoma cells, detected by γ-H2AX, which was completely abrogated by pre-treating cells with ROS scavenger, NAC. Plasma-induced DNA damage in turn may lead to the observed plasma-induced apoptosis. Since plasma is non-thermal, it may be used to selectively treat malignancies.
Starting Page	674
Ending Page	687
Page Count	14
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	39
Issue Number	2
e-ISSN	15739686
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-10-29
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Apoptosis Non-thermal plasma Reactive oxygen species Cancer therapy Plasma medicine Biomedicine general Biomedical Engineering Biophysics and Biological Physics Mechanics Biochemistry
Content Type	Text
Subject	Biomedical Engineering

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