NDLI: Attachment, Spreading, and Adhesion Strength of Human Bone Marrow Cells on Chitosan

Content Provider	Springer Nature Link
Author	Moutzouri, Antonia G. Athanassiou, George M.
Copyright Year	2010
Abstract	The successful integration of an orthopedic implant into bone depends on the mechanisms at the tissue–implant interface and mostly on the osteoblast attachment phenomenon. Chitosan has emerged as an attractive biomacromolecule favoring osseointegration. In this study highly deacetylated chitosan coatings, with roughness of about 1 nm, were bonded to glass surfaces via silane–glutaraldehyde molecules. Human osteoblasts were used to study the development of attachment during the first 60 min. Chitosan favored the number of the attached cells compared to the uncoated surfaces for 30 min seeding time (t s). For t s up to 60 min the attached cell area was almost 210% significantly higher on the chitosan surfaces, indicating an enhanced spreading process. To determine the cell attachment strength, a micropipette aspiration method was used, where the value of the term I = ∫Fdt is representative of the single cell attachment–adhesion procedure and quantitatively reflects the strength evolution during attachment: F equals the detaching force applied on the cell. The results showed higher strength values on the chitosan surfaces. The findings reinforce the favorable environment of the biomacromolecule for the osteoblast and the new approach regarding the quantitatively evaluation of adhesion provides important contribution for the study of cell–material interaction, especially during the crucial first phase of cell attachment.
Starting Page	730
Ending Page	741
Page Count	12
File Format	PDF
ISSN	00906964
Journal	Annals of Biomedical Engineering
Volume Number	39
Issue Number	2
e-ISSN	15739686
Language	English
Publisher	Springer US
Publisher Date	2010-10-26
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Osteoblast Biomacromolecule Micropipette method Cell detachment Cell–material interaction Biomaterials Biochemistry Mechanics Biophysics and Biological Physics Biomedical Engineering Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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