NDLI: Portable bead-based fluorescence detection system for multiplex nucleic acid testing: a case study with Bacillus anthracis

Content Provider	Springer Nature Link
Author	Gravel, Jean François Geissler, Matthias Chapdelaine, Sébastien Boissit, Karel Voisin, Beît Charlebois, Isabelle Poirier Richard, Hugo Pierre Grégoire, Alexandre Boissit, Maurice Bergeron, Michel G. Veres, Teodor Boudreau, Denis
Copyright Year	2013
Abstract	This paper describes the design, functioning and use of a portable detection platform for multiplex nucleic acid testing. The system features a bead-supported DNA hybridization assay performed inside a microfluidic cartridge. Polystyrene particles modified with DNA capture probes are confined in the detection area and exposed to a solution of fluorescently labeled target DNA strands. The cartridge, fabricated from inexpensive thermoplastic polymers, allows for conducting up to eight assays in parallel. The detection instrument is equipped with a pneumatic module and a manifold lid serving as an interface to mediate fluid displacement on the cartridge. The fluorescence signal deriving from each assay is recorded by a semi-confocal fluorescence reader embedded in the detection platform. The compact design of the instrument and its level of integration make it possible to obtain an analytical result in less than 15 min, while only few manual steps need to be performed in between. A proof-of-concept demonstration involving Cy3-labeled, PCR-amplified genomic DNA confirms the ability to detect Bacillus anthracis in a multiplexed single-assay format using lef and capC genes. Limits of quantification are on the order of 1 × 109 copies/μL for lef targets.
Starting Page	1075
Ending Page	1087
Page Count	13
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	16
Issue Number	6
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-11-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biothreat detection DNA hybridization Integration Microfluidic chip Point-of-care testing Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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