NDLI: Separation and concentration of Phytophthora ramorum sporangia by inertial focusing in curving microfluidic flows

Content Provider	Springer Nature Link
Author	Clime, Liviu Li, Kebin Geissler, Matthias Hoa, Xuyen D. Robideau, Gregg P. Bilodeau, Guillaume J. Veres, Teodor
Copyright Year	2016
Abstract	We present an integrated microfluidic system for performing isolation and concentration of Phytophthora ramorum pathogens using a chip whose working principle is based on inertial lateral migration in curving flows. The chip was fabricated from multiple layers of thermoplastic polymers and features an embedded spiral separation channel along with peristaltic microvalves for fluidic operation and process control. A pumping system paired with a fully programmable pressure manifold is used to boost concentration levels by recirculating the sample liquid multiple times through the separation chip, making it possible to reduce sample volumes from 10 to 1 mL or less. The system was calibrated using fluorescent polymer particles with a nominal diameter of 30 µm which is comparable to that of P. ramorum sporangia. The separation process has been shown to be highly effective and more than 99% of the beads can be recovered in the concentrated batch. Experiments conducted with P. ramorum sporangia have shown that a 5.3-fold increase in pathogen content with 95% recovery can be achieved using three subsequent concentration cycles. The utility of the method has been validated by processing a sample derived from infested Rhododendron leaves where a 6.1-fold increase in the concentration of P. ramorum has been obtained after four concentration cycles. Although specifically designed and demonstrated for sporangia of P. ramorum, the method and related design rules can easily be extended to other microbial organisms, effectively supporting bioanalytical applications where efficient, high-throughput separation of target species is of primary concern.
Starting Page	1
Ending Page	13
Page Count	13
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	21
Issue Number	1
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-12-31
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Inertial separation Microfluidic chip Plant pathogen detection Pneumatic pumping Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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