NDLI: Enhanced electrophoresis separation of non-electroactive amino acids on poly(dimethylsiloxane) microchip coupled with direct electrochemical detection on a copper electrode

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Enhanced electrophoresis separation of non-electroactive amino acids on poly(dimethylsiloxane) microchip coupled with direct electrochemical detection on a copper electrode

Microfluidics and Nanofluidics : Volume 11, Issue 1, July 2011

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Enhanced electrophoresis separation of non-electroactive amino acids on poly(dimethylsiloxane) microchip coupled with direct electrochemical detection on a copper electrode

Content Provider	Springer Nature Link
Author	Liang, Ruping Wang, Li Meng, Xiangying Wang, Jingwu Qiu, Jianding
Copyright Year	2011
Abstract	Electrochemical determination of amino acids on a Cu electrode was established as an attractive scheme for non-electroactive amino acids after microchip electrophoresis separation. Five amino acids (arginine, proline, histidine, valine, and serine) achieved efficient separation within 60 s on a titanium dioxide nanoparticles (TiO2 NPs) coated poly(dimethylsiloxane) (PDMS) microchip, and then successfully detected on a Cu electrode in end-channel detection mode. In the slightly basic borate medium, anodic currents occur for amino acids due to their ability to form Cu(II) complexes and thereby enhance the electrochemical dissolution of Cu electrode substrate. The increase of the anodic current measured is proportional to the amino acid concentration added to the solution, and therefore, enables direct detection of non-electroactive amino acids on the Cu electrode. The detection limits (S/N = 3) for arginine, proline, histidine, valine, and serine were measured to be 7, 6, 5, 6, and 5 μM, respectively, with the linear ranges all from 25 to 500 μM. In addition, compared with the native PDMS microchip, resolutions and separation efficiencies of amino acids on the modified microchip were considerably enhanced with the theoretical plate numbers of 8.9 × 103, 6.6 × 104, 4.8 × 104, 5.6 × 104, and 4.4 × 104 plates m−1, respectively. The proposed Cu electrode response demonstrated good reproducibility and stability, with no apparent loss of response for periods as long as 4 weeks.
Starting Page	227
Ending Page	233
Page Count	7
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	11
Issue Number	2
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-04-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Non-electroactive amino acids Microchip electrophoresis Copper electrode Poly(dimethylsiloxane) Biomedical Engineering Industrial Chemistry/Chemical Engineering Engineering Fluid Dynamics
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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