NDLI: Zone electrophoresis of proteins in poly(dimethylsiloxane) (PDMS) microchip coated with physically adsorbed amphiphilic phospholipid polymer

Content Provider	Springer Nature Link
Author	Nii, Kyosuke Sueyoshi, Kenji Otsuka, Koji Takai, Madoka
Copyright Year	2012
Abstract	The zone electrophoresis of protein in poly(dimethylsiloxane) (PDMS) microchip coated with the physically adsorbed amphiphilic phospholipid polymer (PMMSi) was investigated. PMMSi was composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) and 3-(methacryloyloxy) propyltris (trimethylsiloxy) silane (MPTSSi) units in a random fashion. The membrane of PMMSi can be formed on the PDMS surface by a simple and quick dip-coating method. The membrane showed high hydrophilicity and good stability in water, as determined by contact angle measurement, fourier-transformed infrared absorption by attenuated total reflection (ATR-FTIR), and X-ray photoelectron spectroscopy (XPS) analysis. High suppression of protein adsorption to the PDMS surface and reduction in electroosmotic flow (EOF) were achieved by PMMSi coating due to an increase of hydrophilicity, and a decrease of the ζ-potential on the surface of PDMS. For zone electrophoresis, the PMMSi30 containing 30 % hydrophilic MPC was the most suitable molecular design in terms of the stability of the coated membrane on PDMS surface. The average value of EOF mobility of PDMS microchip coated with PMMSi30 was 1.4 × 10−4 cm2 V−1 s−1, and the RSD was 4.1 %. Zone electrophoresis of uranine was further demonstrated with high repeatability and reproducibility. Separation of two FITC-labeled proteins (BSA and insulin) was performed with high efficiency and resolution compared with non-treated PDMS microchip.
Starting Page	951
Ending Page	959
Page Count	9
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	14
Issue Number	6
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-12-04
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microchip electrophoresis PDMS Phospholipid polymer Surface modification Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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